LncRNA FEZF1-AS1 Promotes TGF-β2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04

Figure 2

FEZF1-AS1 promotes TGF-β2-induced SRA01/04 cell migration. SRA01/04 cells were transfected with smart NC, smart silencer, pcDNA3.1, or pcDNA3.1-FEZF1-AS1 with or without TGF-β2 treatment for 48 h. Then, wound healing photos were taken (a), and the wound widths were measured in the FEZF1-AS1 knockdown groups (b) , TGF-β2 plus smart silencer group compared to TGF-β2 plus smart NC group. (c) The wound widths were measured in the FEZF1-AS1 overexpression groups. , pcDNA3.1-FEZF1-AS1 group compared to pcDNA3.1 group. , TGF-β2 plus pcDNA3.1-FEZF1-AS1 group compared to TGF-β2 plus pcDNA3.1 group. (d) Representative photos of the transwell assay are shown. (e) The average number of migrated cells was calculated in the FEZF1-AS1 knockdown groups. , TGF-β2 plus smart silencer group compared to TGF-β2 plus smart NC group. n = 5 in each group. (f) The average number of migrated cells was calculated in the FEZF1-AS1 overexpression groups. , pcDNA3.1-FEZF1-AS1 group compared to pcDNA3.1 group. , TGF-β2 plus pcDNA3.1-FEZF1-AS1 group compared to TGF-β2 plus pcDNA3.1 group. Scale bar = 50 μm in (a) and (c).

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