LncRNA FEZF1-AS1 Promotes TGF-β2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04

Figure 3

FEZF1-AS1 promotes TGF-β2-induced SRA01/04 cell proliferation. (a) SRA01/04 cells were transfected with smart negative control (NC) or smart silencer with or without TGF-β2 treatment for 48 h. FEZF1-AS1 levels in SRA01/04 cells were detected by qRT-PCR. , smart silencer group vs smart NC group. , TGF-β2 plus smart silencer group vs TGF-β2 plus smart NC group. SRA01/04 cells were transfected with smart NC or smart silencer with or without TGF-β2 treatment for 48 h. (b) The viability of HLECs (SRA01/04 cells) was detected by CCK-8 assay. vs TGF-β2 plus smart NC group. (c) SRA01/04 cells were stained with EdU (red) and DAPI (blue). (d) The bar chart shows the average ratio of EdU-stained cells to DAPI-stained cells. , TGF-β2 plus smart silencer group vs TGF-β2 plus smart NC group. (e) SRA01/04 cells were transfected with pcDNA3.1 (empty vector) or pcDNA3.1-FEZF1-AS1 with or without TGF-β2 treatment for 48 h. FEZF1-AS1 levels in SRA01/04 cells were detected by qRT-PCR. , pcDNA3.1-FEZF1-AS1 group vs pcDNA3.1 group. , TGF-β2 plus pcDNA3.1-FEZF1-AS1 group vs TGF-β2 plus pcDNA3.1 group. SRA01/04 cells were transfected with pcDNA3.1 or pcDNA3.1-FEZF1-AS1 with or without TGF-β2 treatment for 48 h. (f) The viability of HLECs (SRA01/04 cells) was detected by CCK-8 assay. , pcDNA3.1-FEZF1-AS1 group vs pcDNA3.1 group. , TGF-β2 plus pcDNA3.1-FEZF1-AS1 group vs TGF-β2 plus pcDNA3.1 group. (g) SRA01/04 cells were stained with EdU (red) and DAPI (blue). (h) The bar chart shows the average ratio of EdU-stained cells to DAPI-stained cells. vs pcDNA3.1 group. vs TGF-β2 plus pcDNA3.1 group. SRA01/04 cells were transfected with smart NC, smart silencer, pcDNA3.1, or pcDNA3.1-FEZF1-AS1 with or without TGF-β2 treatment for 48 h. CDK-2, CDK-4, CDK-6, and cyclin D1 protein levels were detected by western blot and (i) band density analysis (j) , TGF-β2 plus smart silencer group compared to TGF-β2 plus smart NC group. (k) , pcDNA3.1-FEZF1-AS1 group compared to pcDNA3.1 group. , TGF-β2 plus pcDNA3.1-FEZF1-AS1 group compared to TGF-β2 plus pcDNA3.1 group. n = 5 in each group. Scale bar = 50 μm in (c) and (g).

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