LncRNA FEZF1-AS1 Promotes TGF-β2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04

Figure 4

FEZF1-AS1 promotes TGF-β2-induced SRA01/04 cell proliferation and migration via upregulating FEZF1 protein levels. (a) The FEZF1 protein level in the vehicle, TGF-β2-treated, TGF-β2 plus smart NC, TGF-β2 plus smart silencer, TGF-β2 plus pcDNA3.1, and TGF-β2 plus pcDNA3.1-FEZF1-AS1 SRA01/04 cells was detected by western blot. (b) The intensity of the band for FEZF1 was analyzed. , TGF-β2-treated group vs vehicle group. , TGF-β2 plus smart silencer group or TGF-β2 plus pcDNA3.1-FEZF1-AS1 group vs TGF-β2 group. (c) The FEZF1 mRNA levels in SRA01/04 cells transfected with scrambled siRNA, FEZF1 siRNA1, FEZF1 siRNA2, or FEZF1 siRNA3 were detected by qRT-PCR. , , vs scrambled siRNA group. (d) The FEZF1-AS1 levels in SRA01/04 cells transfected with scrambled siRNA or FEZF1 siRNA2 were detected by qRT-PCR. (e) SRA01/04 cells transfected with pcDNA3.1 plus scrambled siRNA, pcDNA3.1 plus FEZF1 siRNA2, pcDNA3.1-FEZF1-AS1 plus scrambled siRNA, or pcDNA3.1-FEZF1-AS1 plus FEZF1 siRNA2 were subjected to EdU ((e) and (g)) and transwell assays ((f) and (h)). , pcDNA3.1 plus FEZF1 siRNA2 group vs pcDNA3.1 plus scrambled siRNA in (g) and (h). , pcDNA3.1-FEZF1-AS1 plus FEZF1 siRNA2 group vs pcDNA3.1-FEZF1-AS1 plus scrambled siRNA group in (g) and (h). n = 5 in each group. Scale bar = 50 μm in (e) and (f).

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