Analysis of TGFBI Gene Mutations in Three Chinese Families with Corneal Dystrophy

Table 1

Sequences of the primers specific for the 17 exons of the TGFBI gene and PCR amplification conditions.

Exon	Primer sequence (5′⟶ 3′)	Annealing temperature (°C)	Product length (bp)
1	F: CGGAGGCGCTCTCACTTCC	62	277
	R: CGAGCCCCGACTACCTGACC
2	F: GGGAGTCATTAAAGTGGGGTGGA	64	290
	R: AGCTTGGTCTCCTGGCTGGTTAC
3	F: CAACTTAGTGGAGAGGGGCCAGA	63	260
	R: CTCTCTCCCACCATTCCCTTCC
4	F: GCCATCCCTCCTTCTGTCTTCTG	62	252
	R: CCTCGGGGAAGTAAGGCAGTTC
5	F: ACTGACACCCTGTCCTTCCTCCT	67	165
	R: AGCCCACACATGGAACAGAAATG
6	F: CTGCTCATCCTTGCTGCTTCTCT	56	248
	R: AGAGTTCCTGCTAGGCCCCTCTT
7	F: TCTGTGGGGAGTGCCAGAGTC	55	238
	R: CAAATGAGGCAGCAGCAGGA
8	F: TGGACCCTGACTTGACCTGAGTC	56	310
	R: AAAGGATGGCAGAAGAGATGGTG
9	F: CCCTGGGGTGGATGAATGATAAA	62	192
	R: GCCTCCAGGGACAATCTAACAGG
10	F: ATTGCAGGAGCACATCTCTCTGG	60	222
	R: GCTTCCCAGGAGCATGATTTAGG
11	F: GCCCCTCGTGGAAGTATAACCAG	55	228
	R: ATCCCACTCCAGCATGACCACT
12	F: GGGCCCTGAGGGATCACTACTTT	56	199
	R: TGACAGGTGACATTTTCTGTGTGTG
13	F: CAGCCTTTGATTTGCAGGACACT	58	195
	R: TGACCAGGCTAATTACCATTCTTGG
14	F: CCAACTGCCACATGAAGAAAAGG	60	280
	R: TGCTCTACCTTTCAACCACTACTCTG
15	F: CCTCTATGGCCCAAACAGAGGAC	57	231
	R: TACCTCTGGTCAAACCTGCCTTT
16	F: ATACAGCAGATGGCAGGCTTGG	55	246
	R: GCCATTGTCATAAGCAGTTGCAG
17	F: ATTGAGGTGTGGAGGAGCATGAC	62	214
	R: TGGGGAGATCTGCACCTATTTGA