Repressed Wnt Signaling Accelerates the Aging Process in Mouse Eyes

Figure 4

sFRP1 causes fibrillary accumulation in the anterior chamber and weakened zonules. (a, b) H&E staining of the control and KR/sFRP1 eyes. Arrow in the highlighted box indicates the fibrillary material in the anterior chamber, as evidenced in KR/sFRP1 eyes but not controls. (c) and (d) are overexposure images to reveal zonular structure (arrows). In the control eyes, the main zonular bundles were straight and compact. In the KR/sFRP1 eyes, the zonular bundles were curly, loose, disorganized, and decorated with granular material. (e) and (f) are confocal scanning images of PAS staining, which confirmed the presence of proteoglycans. Arrows indicate the differences in zonular structure.