ROCK Inhibitor-Induced Promotion of Retinal Pigment Epithelial Cell Motility during Wound Healing

Figure 2

The effect of Y27632 on RPE cell toxicity, cell spreading, and focal adhesion size. (a) Phase-contrast images of RPE cells treated with four different dilutions of Y24632 (0, 10, 100, and 1000 μM) at 1 day after Y27632 administration (scale bar, 50 μm). (b) Histogram showing the cell damage rate of RPE cells treated with four different dilutions of Y27632 at 1 day after Y27632 administration, for each; . (c) F-actin (red), vinculin (green), and DAPI (blue) stained confocal images (left and middle figures) and binarized image (right figure) of untreated RPE cells. (d) F-actin (red), vinculin (green), and DAPI (blue) stained confocal images (left and middle figures) and binarized image (right figure) of Y27632-treated RPE cells. (e) F-actin (red), vinculin (green), and DAPI (blue) stained confocal images (left and middle figures) and binarized image (right figure) of untreated RPE cells. (f) F-actin (red), vinculin (green), and DAPI (blue) stained confocal images (left and middle figures) and binarized image (right figure) of Y27632-treated RPE cells. (g) Histogram showing the F-actin-based cell surface area of RPE cells treated with and without Y27632, for each; . (h) Histogram showing the vinculin size of RPE cells treated with and without Y27632,  cells for each; .