The Petri Dish-N2B27 Culture Condition Maintains RPE Phenotype by Inhibiting Cell Proliferation and mTOR Activation

Figure 4

Expression levels of RPE cell type-specific genes and EMT-related genes in SD rat RPE cells at different time points. (a, b) QPCR analysis of RPE65, CRALBP, bestrophin, N-cadherin, fibronectin, and α-SMA in freshly isolated RPE tissues and in RPE cells cultured in 10% FBS containing medium (cell density: 1,5000 cells/cm²) for 3 days. (c–h) The expressions of RPE65, CRALBP, bestrophin, N-cadherin, fibronectin, and α-SMA genes in the two cell density groups at different time points. Low cell density group: 5,000 cells/cm². High cell density group: 1,5000 cells/cm². Data were expressed as mean ± SD. n = 4 in each group. .

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