Casein Kinase 2-Interacting Protein-1 Alleviates High Glucose-Reduced Autophagy, Oxidative Stress, and Apoptosis in Retinal Pigment Epithelial Cells via Activating the p62/KEAP1/NRF2 Signaling Pathway

Figure 3

CKIP-1 activated the p62/Keap1/Nrf2 signaling pathway. (a) p62 expression was determined by Western blot in different groups. (b) Keap1 expression was determined by Western blot. (c) The expression of Nrf2 in whole cells or in the nucleus and cytosol. Lamin B2 and GAPDH were performed as the loading control of nuclear, whole cells, and cytoplasm, respectively.  < 0.05 and  < 0.001 vs. NG.  < 0.05,  < 0.01, and  < 0.001 vs. HG.  < 0.05,  < 0.01, and  < 0.001 vs. NC. NG, normal glucose (5.5 mM); MG, mannitol (20 mM); HG, high glucose (25 mM); NC, pcDNA3.1 vector negative control; CKIP-1, pcDNA3.1-CKIP-1.

(a)

(b)

(c)