Human Platelets Take up Anti-VEGF Agents

Figure 2

Localization of anti-VEGF agents using immunofluorescence microscopy and immune electron microscopy in human platelets. FITC-labeled anti-VEGF agents (green). Alexa 549-labeled F-actin (red). Overlay of immunofluorescence for anti-VEGF agents and F-actin: (a) ranibizumab, (b) aflibercept, and (c) bevacizumab. (d) Negative control; gold particle (6 nm)-labeled anti-VEGF agents: (e, i) ranibizumab; (f, j) aflibercept; (g, k) bevacizumab; (h., l) negative control. Scale bar represents 1 µm.

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