Human Platelets Take up Anti-VEGF Agents

Figure 3

Colocalization of anti-VEGF agents and VEGF using triple immune fluorescence staining and double immunogold staining in human platelets. FITC-labeled anti-VEGF agents (green) and VEGF (blue); Alexa 549-labeled F-actin (red). Overlay of immunofluorescence for anti-VEGF agents, VEGF, and F-actin: (a) ranibizumab, (b) aflibercept, and (c) bevacizumab. Large gold particle (12 nm)-labeled VEGF and small gold particle (6 nm)-labeled anti-VEGF agents: (d, g) ranibizumab; (e, h) aflibercept; (f, i) bevacizumab. Scale bar represents 1 µm.

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