Activation of Delta-Opioid Receptor Protects ARPE19 Cells against Oxygen-Glucose Deprivation/Reoxygenation-Induced Necroptosis and Apoptosis by Inhibiting the Release of TNF-α

Figure 2

Different durations of reoxygenation have diverse effects on DOR and TNF-α protein levels in ARPE19 cells. (a) TNF-α levels in cell lysates after different reoxygenation times were measured by ELISA (n = 5 per group). (b–e) The protein expression of DOR after different reoxygenation times was examined by Western blotting (n = 3 per group). Quantification of the density values of DOR was normalized to those of β-actin. Data are presented as means ± SEM, and group differences were analyzed by Student’s t-tests. ^#, ^##, ^###, compared with the control group; , OGD24/R24 group vs. OGD24/R36 group.

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