Activation of Delta-Opioid Receptor Protects ARPE19 Cells against Oxygen-Glucose Deprivation/Reoxygenation-Induced Necroptosis and Apoptosis by Inhibiting the Release of TNF-α

Figure 4

The DOR agonist TAN-67 reduced the number of necroptotic and apoptotic ARPE19 cells after OGD24/R36. (a) A Calcein-AM/PI kit was used to distinguish live cells from necroptotic cells. Representative images from left to right: nuclear staining with Hoechst 33258 (blue); calcein-AM staining of live cells (green); PI staining of necroptotic cells (red); merged image. The white square indicates representative cells that are magnified. Scale bar = 100 μm. (b) The bar graph represents the number of PI-positive cells in each group (n = 5 per group). (c) Apoptosis was determined using a TUNEL kit. Representative images from left to right: nuclear staining with Hoechst 33258 (blue); TUNEL of apoptotic cells (green); merged image. The white square indicates representative cells that are magnified. Scale bar = 50 μm. (d) The bar graph represents the number of TUNEL-positive cells in each group (n = 5 per group). Data are presented as means ± SEM, and group differences were analyzed by one-way ANOVA with Fisher’s LSD test. ^### compared with the control group; , compared with the OGD24/R36 group.

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