Circ-ADAM9 Promotes High Glucose-Induced Retinal Pigment Epithelial Cell Injury in DR via Regulating miR-338-3p/CARM1 Axis

Figure 3

Circ-ADAM9 sponged miR-338-3p. (a) The binding sites between circ-ADAM9 and miR-338-3p were shown. (b) miR-338-3p expression was detected by qRT-PCR to assess the transfection efficiency of miR-338-3p mimic. The interaction between circ-ADAM9 and miR-338-3p was verified by dual-luciferase reporter assay (c) and RIP assay (d). (e) The miR-338-3p expression in the serum of DR patients and normal control volunteers was detected by qRT-PCR. (f) Pearson’s correlation coefficient analysis was used to analyze the correlation between circ-ADAM9 and miR-338-3p. (g) QRT-PCR was performed to examine miR-338-3p expression in ARPE-19 cells under HG and NG conditions. (h) Circ-ADAM9 expression was measured by qRT-PCR to evaluate the transfection efficiency of pCD5 circ-ADAM9 overexpression vector. (i) miR-338-3p expression was detected by qRT-PCR in HG-induced ARPE-19 cells transfected with si-circ-ADAM9 or pCD5 circ-ADAM9 overexpression vector. and .

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