The Impact of Different Oxygen Delivery Methods on Corneal Epithelial Repair after Injury

Figure 3

TEM images in each group at 24 h after operation. TEM images of group A: 5,000x (A1), 15,000x (A2), and 50,000x (A3); TEM images of group B: 5,000x (B1), 15,000x B1 (B2), and 50,000x (B3); TEM images of group C: 5,000x (C1), 15,000x (C2), and 50,000x (C3). (1) The microvilli in A and B groups are closely and regularly arranged. The microvilli in group C are absent, and the surface epithelial cells are necrotic (①). (2) There are a lot of glycocalyx on the cell membrane of microvilli in A and B groups, but no glycocalyx is seen in in group C (①, the enlarged picture is indicated by the arrow). (3) Desmosome junction and hemidesmosome junction can be seen in A and B groups; in group (C), the distribution of desmosome junctions is disordered, the number of desmosomes is reduced, the tension filaments are significantly reduced, and necrotic cells can be seen on one side (②). (4) In group B and C, the mitochondria are swollen, endoplasmic reticulum is expanded, and mitochondrial cristae are damaged (③); myeloid corpuscles are visible. (5) The number of corneal epithelium layers in group C is more than that in group A and B under transmission electron microscope (④). (6) The corneal epithelium layers in group C were more than that in group A and B, but the boundary of each stratification is not obvious (⑤).