miR-486-5p Restrains Extracellular Matrix Production and Oxidative Damage in Human Trabecular Meshwork Cells by Targeting TGF-β/SMAD2 Pathway

Figure 5

miR-486-5p regulated H₂O₂-induced injury in TMCs by targeting SMAD2. After H₂O₂-treated TMCs were transfected with NC mimics alone, miR-486-5p mimics alone, miR-486-5p mimics plus pcDNA3.1 alone, or miR-486-5p mimics plus pcDNA3.1/SMAD2, (a, b) mRNA and protein expression of SMAD2 was assessed by RT-qPCR and western blotting, respectively; (c) apoptosis was assessed by flow cytometry; (d) protein expression of cleaved caspase-3/9 was determined by western blotting; (e, f) the SOD and MDA levels were assayed; and (g) expression of collagen I, fibronectin, and laminin was tested by western blotting. ; ; .

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