Novel Mutations Identified in the Chinese Han Population with Keratoconus by Next-Generation Sequencing

Table 2

Sequences of primers used in this study.

Gene	Mutations	Primer name	Fragment size (bp)	Primer sequence
FNDC3B	c.455C > T:p.P152L	6F	417	TGCGTTCTTTCCTTTTGTGTT
		6R		GCTAGGAATTCCCCATGAGTC
COL4A4	c.3636_3637del:p.R1212fs	7F	324	TCCTCATTGCATTTGGAAGGT
		7R		GCAACCAGTTGTTGGTGTCTG
MPDZ	c.5015G > T:p.R1672L	12F	484	GTTCCTTAGTGAGGGGGCTAA
		12R		AATGCCTGTTTTTGACCTTCA
MPDZ	c.3798dupA:p.P1267fs	9F	494	GAGACACAGAGTGGCTGATCC
		9R		ACAAACCCACTGCATTCTTTT
MPDZ	c.28G > A:p.A10T	13F	345	CCTGCTTGGGTGAATGATGTC
		13R		GGGTCCGGCCTACTGTTTTT
DOCK9	c.1940C > T:p.P647L	17F	353	CAAACCTTAAGGGGCTGAGAG
		17R		CCTGCAGTAAAACTCCCATCA
POLG	c.127_128insGGC:p.Q43delinsRQ	11F	425	CAGCTCCACGTCGGGCAAGG
		11R		GCCCAAAGCCAGGTGTTCTGACTCC
IPO5	c.3019G > A:p.V1007I	8F	445	CCCTCTGTTGCCATTCTGTAA
		8R		AGGAAGCACTGTGGAGGAGA
TGFBI	c.624 + 7− > A	19F	402	CAGAGTTGCAAGGACCCATCT
		19R		GCAAAATGTGGGTTCCACAAG