Influence of Light-Emitting Diode-Derived Blue Light Overexposure on Rat Ocular Surface

Figure 4

NF-κB pathway-related proteins in the cornea after blue light irradiation. (a) The production of NF-κB, Iκ-B, IL-6, and TNF-α were evaluated by real-time PCR, with GAPDH as a loading control. After treatment for 28 days, the mRNA levels of NF-κB, TNF-α, and IL-6 were upregulated and significantly higher than those in the control group (, , ). (b–e) The effects of blue light on the activation of NF-κB in corneas were evaluated by Western blot analysis using β-actin as a loading control. (b) After exposure for 28 days, the protein levels of total NF-κB p65 and phosphorylated NF-κB p65 were upregulated and higher than those in the control group. (c, d) The bands showed the statistical analysis of groups by density values. (e) The ratio of phosphorylated NF-κB to total NF-κB showed a significant difference compared with the control group. , .

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