Influence of Light-Emitting Diode-Derived Blue Light Overexposure on Rat Ocular Surface

Figure 7

Effects of blue light on NF-κB activation. (a) Real-time PCR for the expression of cytokines and inflammatory factors. The production of IL-6 and TNF decreased significantly after PDTC treatment (), while the expression of Iκ-B upregulated significantly (). (b) Effects of blue light on the activation of NF-κB in corneas are evaluated by Western blot analysis using β-actin as a loading control. (c, d) PDTC treatment decreased the expression of total NF-κB p65 and phosphorylated NF-κB p65 (). The ratio of phosphorylated NF-κB p65 to total NF-κB p65 showed no significant difference.

(a)

(b)

(c)

(d)

(e)