Proinflammatory Cytokines Trigger the Onset of Retinal Abnormalities and Metabolic Dysregulation in a Hyperglycemic Mouse Model

Figure 4

Focal ERG a-wave and b-wave amplitude and implicit time two days after intravitreal injection. (a) Average a-wave amplitude. (b) Average b-wave amplitude. (c) Average a-wave implicit time. (d) Average b-wave implicit time. (e) Summed OP amplitude. Two days postintravitreal injection, there was a significant reduction in a-wave amplitude in the hyperglycemic mice at 3.2 log cd.s/m² (represented by the green asterisk) compared to control, while the hyperglycemic mice with intraocular cytokines showed further reduction of the a-wave amplitude at 2.6 and 3.2 log cd.s/m² (represented by the red asterisk) compared to control. Likewise, there was a significant reduction in b-wave amplitude in the hyperglycemic mice at 2.6 log cd.s/m² (represented by the green asterisk) compared to control, while the hyperglycemic mice with intraocular cytokines showed further reduced b-wave amplitude at 2.0, 2.6, and 3.2 log cd.s/m² (represented by the red asterisk) compared to control. There were no significant changes in the OP amplitude in the experimental group compared to control mice. Statistical significance was determined using two-way ANOVA followed by post hoc Dunnett’s multiple comparison tests. Values represent mean ± SEM (n = 6). ^∗ < 0.05; ^∗∗ < 0.01; ^∗∗∗ < 0.001.

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