Proinflammatory Cytokines Trigger the Onset of Retinal Abnormalities and Metabolic Dysregulation in a Hyperglycemic Mouse Model

Figure 5

Representative focal ERG response two days after intravitreal injection. Focal ERG response in control mice (a), control mice intravitreally injected with cytokines (b), hyperglycemic mice without intraocular cytokines (c), and hyperglycemic mice with intraocular cytokines (d). a′, b′, c′, and d′ are the OPs within the corresponding ERG response at the highest light intensity (3.2 log cd.s/m2). The a-wave, b-wave, and the OP response of the control mice to intraocular cytokines were comparable to the control mice. While the b-wave amplitude of the hyperglycemic mice without intravitreal cytokines appeared to be reduced (indicated in green arrow), the hyperglycemic mice with intraocular cytokines had visibly reduced a-wave and b-wave amplitudes at 2, 2.6, and 3.2 log cd.s/m2 (indicated in red arrows). The control and hyperglycemic mice injected with proinflammatory cytokines appeared to have low OP response (b′ and d′) compared to control mice. The vertical grey dashed line at 50 ms represents the time point at which the white-light stimulus is presented.