Trimethylamine-N-Oxide Promotes High-Glucose-Induced Dysfunction and NLRP3 Inflammasome Activation in Retinal Microvascular Endothelial Cells

Figure 2

TMAO promotes cell proliferation induced by high glucose in human retinal microvascular endothelial cells. HRMECs were treated with NG (normal glucose, D-glucose 5.5 mM) + TMAO (0 μM) and HG (high glucose, D-glucose 30 mM) + TMAO (0, 1.25, 2.5, 5, 10, 20, 50, 100 μM) for 72 h. (a) OD450 values were read after 2 h of adding CCK8 solution. vs. NG + TMAO (0 μM), n = 4. HRMECs were treated with NG (D-glucose, 5.5 mM), NG + TMAO (5 μM), HG (D-glucose, 30 mM), and HG + TMAO (5 μM) for 72 h. (b) OD450 values were read after 2 h of adding CCK8 solution. vs. NG; ^#vs. NG + TMAO, n = 4.

(a)

(b)