Mutational Profile and Retinal Phenotypes of <i>PCARE</i>-Related Cone-Rod Dystrophies in a Mexican Cohort

López-Rodríguez, Víctor R.; Arce-González, Rocío; Martínez-Aguilar, Alan; Rodríguez-López, Carlos E.; Groman-Lupa, Sergio; Neria-González, M. Isabel; Rodríguez-Uribe, Genaro; Zenteno, Juan C.

doi:https://doi.org/10.1155/2024/4003914

Journal of Ophthalmology

On this page

Abstract Introduction Materials and Methods Results Discussion Data Availability Ethical Approval Consent Conflicts of Interest Acknowledgments Supplementary Materials References Copyright Related Articles

Research Article | Open Access

Volume 2024 | Article ID 4003914 | https://doi.org/10.1155/2024/4003914

Mutational Profile and Retinal Phenotypes of PCARE-Related Cone-Rod Dystrophies in a Mexican Cohort

Víctor R. López-Rodríguez,¹Rocío Arce-González,¹Alan Martínez-Aguilar,²Carlos E. Rodríguez-López,³Sergio Groman-Lupa,⁴M. Isabel Neria-González,⁵Genaro Rodríguez-Uribe,^4,6and Juan C. Zenteno^1,7,8

Academic Editor: Akio Oishi

Received20 Dec 2023

Revised31 Jan 2024

Accepted24 Feb 2024

Published04 Mar 2024

Abstract

Purpose. The aim of the study is to describe the genotype and phenotype of a Mexican cohort with PCARE-related retinal disease. Methods. The study included 14 patients from 11 unrelated pedigrees with retinal dystrophies who were demonstrated to carry biallelic pathogenic variants in PCARE. Visual assessment methods included best corrected visual acuity, color fundus photography, Goldmann visual field test, kinetic perimetry, dark/light adapted chromatic perimetry, full-field electroretinography, autofluorescence imaging, and spectral domain-optical coherence tomography imaging. Genetic screening was performed either by gene panel sequencing or by exome sequencing. Results. According to the results of multimodal imaging and functional tests, all 14 patients were diagnosed with cone-rod dystrophy. Six different PCARE pathogenic alleles were identified in our cohort, including three novel mutations: c.3048_3049del (p.Tyr1016), c.3314_3315del (p.Ser1105), and c.551A > G (p.His184Arg). Notably, alleles p.His184Arg, p.Arg613, and p.Arg984 were present in 18 of the 22 (82%) PCARE alleles from probands in our cohort. Conclusion. Our work expands the PCARE mutational profile by identifying three novel pathogenic variants causing retinal dystrophy. While phenotypic variations occurred among patients, a cone-rod dystrophy pattern was observed in all affected individuals.

1. Introduction

Inherited retinal degenerations (IRDs) are a heterogeneous group of genetic disorders characterized by progressive dysfunction of neuroretinal cells and/or the retinal pigment epithelium. IRDs have a combined prevalence of approximately one case per 2,000 individuals [1] and are considered the most common cause of blindness in the working-age population [2]. IRDs are a paradigmatic example of diseases with phenotypic and genotypic variabilities, as their age of onset, severity of clinical manifestations, and rate of visual loss are considerably variable among individuals and as mutations in over 280 genes can result in the disease [3, 4]. IRD-related genes participate in a myriad of cellular processes that are essential for the structural and functional integrity of retinal tissue [5]. Genetic screening of individuals with IRDs is a crucial procedure for accurate diagnosis, prognosis, and management of patients, and it has been tremendously improved in recent years by the widespread incorporation of next-generation sequencing (NGS) techniques [6, 7].

Pathogenic variants in PCARE, a gene located at 2p23.2 and previously known as C2orf71, has been recently demonstrated in IRDs [8, 9]. Recessive loss-of-function variants in this gene have been reported in subjects with different retinal phenotypes involving both rods and cones, namely retinitis pigmentosa (RP) and cone-rod dystrophy (CRD) [10–13]. PCARE-related IRD is associated with wide variation in terms of age of onset and progression rate of retinal damage with vision loss typically beginning in the second or third decade of life; about half of the patients also present with nyctalopia at the time of consultation, and vision can be preserved until the fifth or sixth decade [11]. Fundus appearance is particularly variable, ranging from early-onset maculopathy to changes resembling retinitis pigmentosa [11]. The prevalence of PCARE-related IRD is currently unknown, but it is expected to vary from 1% as observed in a French cohort [14] to 15% in a Swiss population [10].

To date, more than 30 PCARE pathogenic variants have been recognized in patients suffering from diverse retinal phenotypes, most of them from European and Asian ethnicities [15, 16]. The majority of these variants correspond to single nucleotide variants or indels that generate premature stop codons or frameshifts [17].

The aim of this study was to describe the phenotypes and disease-causing variants in a cohort of PCARE-related IRD patients from Mexico. Our results add to the knowledge of the clinical and molecular spectrum of retinal dysfunction due to PCARE mutations.

2. Materials and Methods

2.1. Study Population

The study corresponded to a retrospective and descriptive case series comprising 14 affected patients with retinal disease who were demonstrated to carry causative variants in PCARE; all included patients were of Mexican origin. The study was approved by the Institutional Review Board of the Institute of Ophthalmology “Conde de Valenciana” in Mexico City. All procedures adhered to the tenets of the Declaration of Helsinki, and written informed consent was obtained from the participants.

2.2. Clinical Data

Medical records were reviewed to collect clinical data and participants underwent a complete eye examination, including visual acuity testing, ultrawide fundus photography, Goldmann visual field kinetic perimetry, dark/light adapted chromatic perimetry (Metrovision, MonCvONE, France), autofluorescence imaging (FAF), spectral domain-optical coherence tomography (SD-OCT) (Spectralis; Heidelberg Engineering, Heidelberg, Germany), and full-field electroretinography (ffERG) (Metrovision, MonPackONE, France). Mean deficits in chromatic perimetry scores were calculated using an internal database of values from normal Mexican population. The ERG protocol complied with the standards of the International Society for Clinical Electrophysiology of Vision.

2.3. Genetic Analysis

PCAREdisease-causing mutations were identified by either gene panel (patients #1–9, 14) or exome sequencing (ES) (patients #10–13). Briefly, genomic DNA (gDNA) was extracted from peripheral blood leukocytes using the QIAamp DNA Blood Mini Kit (Qiagen, Germany), following the manufacturer’s protocol. In patients #1–9, and 14 gDNA was enriched for targeted regions using a hybridization-based protocol and sequenced using Illumina technology. Sequence analysis and deletion/duplication testing were performed on 298 genes included in the Invitae Inherited Retinal Disorders Panel (Invitae, San Francisco, CA). Target regions were sequenced with ≥50x depth, and reads were aligned to the GRCh37/Hg19 reference sequence. In patients #10–13, ES was performed at 3Billion, Inc. (Seoul, South Korea). DNA library preparation was performed using the IDT xGen Exome Research Panel v2.0 kit (Integrated DNA Technologies, Coralville, Iowa, USA), and sequenced on NovaSeq 6000 (Illumina, San Diego, CA, USA). The mean depth-of-coverage was 140x with a minimum of 98.5% of the targeted region covered at 30x. The base call (BCL) sequence files generated by NovaSeq 6000 were converted and demultiplexed to FASTQ files using bcl2fastq v2.20.0.422. Sequence reads in the FASTQ files were aligned to the human reference genome (GRCh37/hg19) using BWA-mem 0.7.17 to generate BAM files. BAM files were processed following the GATK best practices (GATK v.3.8) for variant calling to generate VCF files. Exome sequencing data annotation and variant filtration were performed using the Franklin platform (Genoox, Palo Alto, CA). The designation of pathogenic or likely pathogenic variants was performed according to the American College of Genetics and Genomics (ACMG) guidelines. In silico analysis and visualization of residue conservation and position at the protein level were performed using Jalview (jalview.org) and ConSurf-DB (consurfdb.tau.ac.il), respectively, in relevant missense variants. Variants of clinical significance were confirmed and segregated in families by Sanger sequencing. Primers sequences and PCR conditions are available on request.

3. Results

3.1. Patients’ Summary

A total of 14 patients pertaining to 11 unrelated families suffering from PCARE-related retinal dystrophies were included in the analysis. Tables 1 and 2 present their demographic and clinical data; two families (II and V) with more than one affected subject were ascertained. The cohort included eight males (57%) and six females. The mean age of symptoms onset was 17.2 years (±11.05, range 5–36), and the mean age upon clinical examination was 36 years (±11.80, range 16–54). Family VI reported a history of consanguinity, while family IX reported endogamy.

3.2. Ocular Phenotypes

All patients had clinical data from at least one ophthalmologic examination, which revealed a tendency towards symmetric affectation (Tables 1 and 2). The symptoms at the onset of the disease were nyctalopia (8/14, 57%), vision loss (3/14, 21%), photophobia (2/14, 14%), and photopsia (1/14, 7%). Visual acuity at examination ranged from 0.09 logMAR to light perception, with 10 out of 13 available patients having in both eyes a logMAR value of 0.50 or worse. Funduscopic common findings included a tessellated appearance, hypopigmented dotting, optic disc pallor, and vessel attenuation (Figure 1). Atrophic maculopathy was observed in six patients (ID# 2, 3, 6, 11, 12, and 14). Chromatic perimetry (available in four patients, #7–9, 11) showed a reduction in the response of both rod and cone systems; photopic mean deficits ranged from 12.63 dB to 20.14 dB, and the scotopic mean deficits ranged from 20.08 dB to 41.9 dB. Five patients (ID# 2, 3, 6, 12, and 13) with low visual acuity or poor fixation were unable to perform a chromatic perimetry test, so a full-field stimulus threshold test (FST) was completed instead. The FST showed a reduced response under scotopic conditions and a more reduced response under photopic conditions in all these patients (Table 3). Goldmann perimetry results were available in seven patients. Preservation of a central island of vision was noted in four patients (ID# 2, 3, 8, 10); additionally, patient #8 had a nasal island of vision in the periphery. In the youngest patient (ID# 7), the visual field was normal using V4e stimulus, while a perifoveal relative scotoma with an augmented peripapillary scotoma was found in the OD using the I4e stimulus.

In 11 patients in whom FAF test was performed, the most frequent findings were hypo-AF with a nummular or mottling pattern in the periphery (ID# 2, 3, 6–14); hyper-AF foveal dot (ID# 2, 3, 7–13); hypo-AF in the central macular area (ID# 2, 3, 6, 9–14); peripapillary hypo-AF (ID# 2, 3, 6, 9, 12, 13); and hyper-AF macular ring surrounding the mentioned hypo-AF macular area (ID# 3, 7–10, 14) (Figure 1). All ten patients who underwent full-field ERG had an abolished response in scotopic, mesopic, and photopic conditions, with the exception of patient #7 who had a subnormal scotopic and mesopic response in addition to an abolished photopic response (see supplementary Table (available here)).

SD-OCT images, available in 11 patients, demonstrated loss of outer retinal layers to different degrees in all of them, preservation of the ellipsoid zone (EZ) in four (ID# 7, 8, 10, 13), and severely atrophic damage with loss of retinal architecture in five patients (ID# 2, 3, 6, 11, and 12). Outer retinal tubulations (ORT) appeared in three patients (ID# 2, 3, 12) (Figure 1). According to multimodal imaging and functional assessment, all 14 patients were diagnosed with CRD.

3.3. Genetic Findings

All affected individuals carried biallelic PCARE pathogenic alleles, including a total of 6 distinct gene variants (Table 4). Pathogenic alleles included four nonsense (two novel: p.Tyr1016 and p.Ser1105), one frameshift, and one novel missense variant (p.His184Arg). Notably, three variants (p.His184Arg, p.Arg613, and p.Arg984) were present in 18 of the 22 PCARE alleles from the 11 probands (82%). Segregation analysis confirmed compound heterozygosity in four probands while a homozygous status was confirmed in the other seven. Based on the American College of Medical Genetics and Genomics criteria for variant assessment, all five identified null variants were classified as pathogenic, while the single identified novel missense variant (p.His184Arg) was classified as likely pathogenic. Of note, the p.His184Arg variant affects a highly conserved residue (GERP: 5.52) (Figure 2) and has an extremely low frequency (gnomAD 0.0004%), and in silico molecular analysis with different tools predicts a deleterious effect (SIFT: 0, PolyPhen-2: 1, VARITY: 0.92).

Figure 2

In silico analysis of the novel p.His184Arg variant in PCARE. (a) Close up of the predicted protein structure of PCARE, the p.His184 residue is located in a predicted helical coiled coil domain. (generated on ConSurf) (b) Protein sequence alignment of PCARE orthologs among different species; the red mark delimitates the p.His184 residue. (generated on Jalview) (c) Sanger sequencing chromatogram showing the c.551A > G (p.His184Arg) variant in the genomic DNA of one heterozygous patient.

4. Discussion

In the present study, we describe in depth the clinical and genetic features of 14 patients, from 11 different pedigrees, with biallelic disease-causing PCARE variants. To our knowledge, this is one of the largest cohorts of PCARE-related IRDs reported to date and the first to include Latin American patients, a highly underrepresented population in published IRDs cohorts.

PCARE stands for photoreceptor cilium actin regulator, which is the name given to the protein product. As its name indicates, it is specifically expressed in the retina [8], and there is evidence showing its localization in the connecting cilium of photoreceptor cells where it participates, along with other proteins (e.g., WASF3), in actin dynamics for the expansion of the ciliary membrane, an important process for outer segment development and homeostasis [18–20].

In our cohort, the clinical findings showed a generalized photoreceptor disorder with early macular involvement, compatible with a CRD pattern. The clinical diagnosis of CRD was established mainly based on the results of chromatic perimetry, since there was a relatively better response under scotopic conditions in all the patients. Early macular atrophy and prominent cone-system deterioration have been mentioned in several reported cases of PCARE retinopathy, usually co-occurring with manifestations of rod-system deterioration [9–11, 14].

The descriptive data obtained from this cohort show, in general terms, a variable expression of the disease, including a variable age at onset ranging from the first to the fourth decade of life, and heterogeneity in the severity of visual symptoms. Indeed, nyctalopia as the first reported symptom in some patients is unexpected for a CRD diagnosis, and this could be the result of early and severe damage in regions with a high density of rods, including some regions of the macula, along with cone damage. All of our patients presented visual loss to some degree, progressing to light perception vision after the fourth decade of life; as expected, older individuals exhibited worse visual acuity in our cohort. Notably, clinical variation appeared to be less marked among subjects pertaining to the same family than among patients from unrelated pedigrees.

ORTs were observed on OCT images from three patients in our cohort. These are tubular retinal structures formed in the outer nuclear layer by reorganization of photoreceptors in different stages of degeneration, Müller cells and RPE cells [21]. These structures have been associated with a variety of retinal conditions, such as age-related macular degeneration, diabetic retinopathy, choroidopathies, and retinal dystrophies [22, 23]. Our observation of ORTs in PCARE-related IRD is consistent with previous reports [11, 15], and notably, the three subjects presented the ORTs in both eyes. In two cases, asymmetric ORTs in terms of their size were observed and, remarkably, in both cases, the eye with the larger ORTs presented better visual acuity (Table 1). Interestingly, a previously reported PCARE-related IRD case series described three patients with ORTs who appeared to have better visual acuity than the rest of the cohort [11]. Although the clinical significance of ORTs is controversial, some authors have recognized their potential use as clinical biomarkers of prognosis [24, 25].

The genetic characterization of our cohort identified a total of six different PCARE pathogenic variants, including three novel mutations. Although all the included families were apparently unrelated, certain PCARE variants had a noticeably higher frequency than others. Interestingly, two of these common variants, p.Arg613 and p.Arg984, have been reported in patients of Korean [11] and French [14] ethnicities, respectively, suggesting that these could be recurrent gene variations. In contrast, the p.His184Arg missense variant, observed in 4 out of 22 probands’ alleles, had not been previously reported. While a possible explanation for this high frequency is a founder mutation effect in our population, additional haplotype analyses are required to confirm this hypothesis.

In accordance with previous reports, our results indicate that the majority of IRD-related PCARE variants are predicted null variants. Thus, five different null variants and a single missense variant were characterized in our cohort, with all six having a predicted loss-of-function effect. Of note, five out of six variants identified here introduce premature termination codons (PTCs), and according to the canonical rules known for nonsense-mediated mRNA decay activation [26], all of them are predicted to activate the mechanism leading to transcript degradation. On the other hand, mutation modeling shows that the novel p.His184Arg variant affects a highly conserved residue of the PCARE protein (Figure 2), located in a likely structured region corresponding to a helical coiled coil domain, which is predicted to have an important role in the function of the protein [18, 19]. Previously characterized pathogenic PCARE missense variants as p.Ile201Phe [8] and p.Cys599Arg [27] also affect relatively conserved residues. Even so, a clear genotype-phenotype correlation was not established in our cohort.

In conclusion, we report one of the largest cohorts of PCARE-related retinal dystrophy, and the first that includes Latino American population. Our results support that CRD is the main phenotype related to PCARE defects and confirm the association of ORT to the disease, an OCT finding that could serve as a clinical biomarker of the disorder. We also expand the mutational spectrum of PCARE with the report of three novel disease-causing variants.

Data Availability

All the clinical and molecular data that support the findings of this study are available from the corresponding author upon reasonable request.

Ethical Approval

The study was approved by the Institutional Review Board of the Institute of Ophthalmology “Conde de Valenciana” in Mexico City. All procedures adhered to the tenets of the Declaration of Helsinki.

Patients signed informed consent regarding publishing their data and photographs.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

Acknowledgments

The authors would like to thank all the participants involved in this study. This work was partially supported by CONAHCYT, Grant No. 568454.

Supplementary Materials

Supplementary Table: Full Field ERG Raw Data showing microvoltage values in analyzed individuals. (Supplementary Materials)

References

A. Rattner, H. Sun, and J. Nathans, “Molecular genetics of human retinal disease,” Annual Review of Genetics, vol. 33, no. 1, pp. 89–131, 1999.
View at: Publisher Site | Google Scholar
R. C. Heath Jeffery, S. A. Mukhtar, I. L. McAllister, W. H. Morgan, D. A. Mackey, and F. K. Chen, “Inherited retinal diseases are the most common cause of blindness in the working-age population in Australia,” Ophthalmic Genetics, vol. 42, no. 4, pp. 431–439, 2021.
View at: Publisher Site | Google Scholar
J. M. Ellingford, R. B. Hufnagel, and G. Arno, “Phenotype and genotype correlations in inherited retinal diseases: population-guided variant interpretation, variable expressivity and incomplete penetrance,” Genes, vol. 11, p. 1274, 2020.
View at: Publisher Site | Google Scholar
RetNet, “Retinal information network,” 2023, https://web.sph.uth.edu/RetNet/.
View at: Google Scholar
A. Manley, B. I. Meshkat, M. M. Jablonski, and T. J. Hollingsworth, “Cellular and molecular mechanisms of pathogenesis underlying inherited retinal dystrophies,” Biomolecules, vol. 13, no. 2, p. 271, 2023.
View at: Publisher Site | Google Scholar
A. Dockery, L. Whelan, P. Humphries, and G. J. Farrar, “Next-generation sequencing applications for inherited retinal diseases,” International Journal of Molecular Sciences, vol. 22, no. 11, p. 5684, 2021.
View at: Publisher Site | Google Scholar
A. C. Britten-Jones, S. A. Gocuk, K. L. Goh, A. Huq, T. L. Edwards, and L. N. Ayton, “The diagnostic yield of next generation sequencing in inherited retinal diseases: a systematic Review and meta-analysis,” American Journal of Ophthalmology, vol. 249, pp. 57–73, 2023.
View at: Publisher Site | Google Scholar
D. Y. Nishimura, L. M. Baye, R. Perveen et al., “Discovery and functional analysis of a retinitis pigmentosa gene, C2ORF71,” The American Journal of Human Genetics, vol. 86, no. 5, pp. 686–695, 2010.
View at: Publisher Site | Google Scholar
R. W. Collin, C. Safieh, K. W. Littink et al., “Mutations in C2ORF71 cause autosomal-recessive retinitis pigmentosa,” The American Journal of Human Genetics, vol. 86, no. 5, pp. 783–788, 2010.
View at: Publisher Site | Google Scholar
A. Tiwari, A. Bahr, L. Bähr et al., “Next generation sequencing based identification of disease-associated mutations in Swiss patients with retinal dystrophies,” Scientific Reports, vol. 6, no. 1, Article ID 28755, 2016.
View at: Publisher Site | Google Scholar
C. Gerth-Kahlert, A. Tiwari, J. V. M. Hanson et al., “C2orf71 mutations as a frequent cause of autosomal-recessive retinitis pigmentosa: clinical analysis and presentation of 8 novel mutations,” Investigative Ophthalmology & Visual Science, vol. 58, no. 10, pp. 3840–3850, 2017.
View at: Publisher Site | Google Scholar
E. Boulanger-Scemama, S. Mohand-Saïd, S. El Shamieh et al., “Phenotype analysis of retinal dystrophies in light of the underlying genetic defects: application to cone and cone-rod dystrophies,” International Journal of Molecular Sciences, vol. 20, no. 19, p. 4854, 2019.
View at: Publisher Site | Google Scholar
B. Bocquet, N. A. Marzouka, M. Hebrard et al., “Homozygosity mapping in autosomal recessive retinitis pigmentosa families detects novel mutations,” Molecular Vision, vol. 19, pp. 2487–2500, 2013.
View at: Google Scholar
I. Audo, M. E. Lancelot, S. Mohand-Saïd et al., “Novel C2orf71 mutations account for ∼1% of cases in a large French arRP cohort,” Human Mutation, vol. 32, no. 4, pp. E2091–E2103, 2011.
View at: Publisher Site | Google Scholar
R. Serra, M. Floris, A. Pinna, F. Boscia, F. Cucca, and A. Angius, “Novel mutations in c2orf71 causing an early onset form of cone-rod dystrophy: a molecular diagnosis after 20 years of clinical follow-up,” Molecular Vision, vol. 25, pp. 814–820, 2019.
View at: Google Scholar
L. Yang, H. Cui, X. Yin et al., “Dependable and efficient clinical molecular diagnosis of Chinese RP patient with targeted exon sequencing,” PLoS One, vol. 10, Article ID e0140684, 2015.
View at: Publisher Site | Google Scholar
M. Zufiaurre-Seijo, J. García-Arumí, and A. Duarri, “Clinical and molecular aspects of C2orf71/PCARE in retinal diseases,” International Journal of Molecular Sciences, vol. 24, no. 13, Article ID 10670, 2023.
View at: Publisher Site | Google Scholar
J. C. Corral-Serrano, I. J. C. Lamers, J. van Reeuwijk et al., “PCARE and WASF3 regulate ciliary F-actin assembly that is required for the initiation of photoreceptor outer segment disk formation,” Proceedings of the National Academy of Sciences of the U S A, vol. 117, no. 18, pp. 9922–9931, 2020.
View at: Publisher Site | Google Scholar
T. A. V. Afanasyeva, Y. T. Schnellbach, T. J. Gibson, R. Roepman, and R. W. J. Collin, “PCARE requires coiled coil, RP62 kinase-binding and EVH1 domain-binding motifs for ciliary expansion,” Human Molecular Genetics, vol. 31, no. 15, pp. 2560–2570, 2022.
View at: Publisher Site | Google Scholar
J. C. Corral-Serrano, M. Messchaert, M. Dona et al., “C2orf71a/pcare1 is important for photoreceptor outer segment morphogenesis and visual function in zebrafish,” Scientific Reports, vol. 8, no. 1, p. 9675, 2018.
View at: Publisher Site | Google Scholar
R. Dolz-Marco, K. M. Litts, A. C. S. Tan, K. B. Freund, and C. A. Curcio, “The evolution of outer retinal tubulation, a neurodegeneration and gliosis prominent in macular diseases,” Ophthalmology, vol. 124, no. 9, pp. 1353–1367, 2017.
View at: Publisher Site | Google Scholar
S. A. Zweifel, M. Engelbert, K. Laud, R. Margolis, R. F. Spaide, and K. B. Freund, “Outer retinal tubulation: a novel optical coherence tomography finding,” Archives of Ophthalmology, vol. 127, no. 12, pp. 1596–1602, 2009.
View at: Publisher Site | Google Scholar
I. Z. Braimah, S. Dumpala, and J. Chhablani, “Outer retinal tubulation in retinal dystrophies,” Retina, vol. 37, no. 3, pp. 578–584, 2017.
View at: Publisher Site | Google Scholar
A. Arrigo, E. Aragona, O. Battaglia et al., “Outer retinal tubulation formation and clinical course of advanced age-related macular degeneration,” Scientific Reports, vol. 11, no. 1, Article ID 14735, 2021.
View at: Publisher Site | Google Scholar
Y. Wang, J. Chen, J. Sun et al., “Shanghai Inherited Retinal Diseases (SHIRD) Study Group. Outer retinal tubulation in Bietti crystalline dystrophy associated with the retinal pigment epithelium atrophy,” Retina, vol. 43, no. 4, pp. 659–669, 2023.
View at: Publisher Site | Google Scholar
E. Nagy and L. E. Maquat, “A rule for termination-codon position within intron-containing genes: when nonsense affects RNA abundance,” Trends in Biochemical Sciences, vol. 23, no. 6, pp. 198-199, 1998.
View at: Publisher Site | Google Scholar
M. G. D. Pozo, C. Méndez-Vidal, N. Bravo-Gil et al., “Exome sequencing reveals novel and recurrent mutations with clinical significance in inherited retinal dystrophies,” PLoS One, vol. 9, no. 12, Article ID e116176, 2014.
View at: Publisher Site | Google Scholar

Copyright

Copyright © 2024 Víctor R. López-Rodríguez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PDF Download Citation

Download other formats

Order printed copies

Views

109

Downloads

115

Citations