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Journal of Osteoporosis
Volume 2012, Article ID 639427, 7 pages
http://dx.doi.org/10.1155/2012/639427
Research Article

Effects of Kalsis, A Dietary Supplement, on Bone Metabolism in the Ovariectomized Rats

1Bioquímica Investigación, Instituto de Investigación Sanitaria, Fundación Jiménez Díaz, Avenida Reyes Católicos 2, 28040 Madrid, Spain
2Internal Medicine, Instituto de Investigación, Sanitaria Fundación Jiménez Díaz, 28040 Madrid, Spain
3Trabeculae Technology Based Firm, Technological Park of Galicia, 32900 Ourense, Spain
4Traumatology and Orthopedic Surgery, University Hospital Complex of Santiago de Compostela, 15706 Santiago de Compostela, Spain
5Instituto Nacional de Endocrinología, 10400 La Habana, Cuba

Received 4 June 2012; Revised 1 August 2012; Accepted 11 September 2012

Academic Editor: Hope Weiler

Copyright © 2012 Mercedes Montero et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We studied the ability of Kalsis, a food supplement that contains selenium, citric acid, and vitamin E, to prevent the effects of ovariectomy on bone loss. Six-month-old, Wistar female rats were studied. Groups ( ): SHAM: sham-operated rats; OVX: ovariectomized rats, treated with vehicle; OVX + Kalsis: ovariectomized rats treated with Kalsis (25 mg/kg/day) for 3 months. Bone mineral density (BMD) was determined by DXA in lumbar spine and femur. Computerized microtomography ( CT) in femur and serum osteocalcin (BGP), aminoterminal propeptide of procollagen I (PINP), β-isomer of carboxyterminal telopeptide of collagen I (CTX), and 5b isoenzyme of tartrate-resistant acid phosphatase (TRAP) were performed. Treatment with Kalsis prevented BMD loss in OVX group. CT showed a decrease in BV/TV, and trabecular number, and an increase in trabecular separation in OVX rats. Kalsis administration attenuated partially bone loss observed by CT due to ovariectomy. BGP, PINP, and the resorption index (CTX/TRAP) were increased in OVX group. Treatment with Kalsis maintained this increase. The mechanism of action of this supplement is not through a decrease in bone remodelling rate. The antioxidant action of this food supplement, due to the synergism of all its components, as a cause of its beneficial effect is suggested.