Effects of Kalsis, A Dietary Supplement, on Bone Metabolism in the Ovariectomized Rats

Table 3

3D trabecular microarchitecture analysis of femur by microtomography in rats which were sham operated (SHAM), ovariectomized (OVX), or ovariectomized and treated with kalsis (OVX + K25). The parameters studied are bone volume/total volume (BV/TV; %), trabecular number (Tb.N; 1/mm), trabecular thickness (Tb.Th; mm), trabecular separation (Tb.Sp; mm), and trabecular pattern factor (Tb.Pf; 1/mm). Female Wistar rats, 6 months old at the beginning of the study, ovariectomized (OVX) or sham-operated (SHAM) and randomized in the following groups: SHAM group (SHAM) treated with vehicle for 3 months; ovariectomized group (OVX) also treated with vehicle for 3 months; ovariectomized group treated with Kalsis at a dose of 25 mg/kg/day, for 3 months (OVX + K25). Statistical significance 𝑃<0.05: a vresus SHAM; b versus OVX.

	SHAM	OVX	OVX + K25
BV/TV (%)	32.11±6.68	13.47±4.88^a	20.21±4.15^a,b
Tb.N (1/mm)	4.55±0.80	1.82±0.53^a	2.24±0.15^a,b
Tb.Th (mm)	0.073±0.004	0.072±0.007	0.090±0.020^a,b
Tb.Sp (mm)	0.152±0.036	0.499±0.127^a	0.405±0.053^a,b
Tb.Pf (1/mm)	−5.571±5.521	7.575±5.411^a	2.414±3.882^a,b