High Folic Acid Intake during Pregnancy Lowers Body Weight and Reduces Femoral Area and Strength in Female Rat Offspring

Table 1

Composition of diets.

Component; (g/kg powdered diet)	RFol diet; (AIN-93G)	HFol diet; (modified AIN-93G)
Cornstarch^1	529.5	529.5
Casein (>85% protein)^2	200.0	200.0
Sucrose (added)^3	100.0*	99.982*
Sucrose (from mineral mix)	21.4	21.4
Sucrose (from vitamin mix)	9.75	9.75
Sucrose (from folate mix)	0.0*	0.018*
Fat4†	70.0	70.0
Fiber (cellulose)	50.0	50.0
Mineral mix	13.6	13.6
Vitamin mix	0.25	0.25
Folic acid (from vitamin mix)	0.002	0.002
Folic acid (from folate mix)	0.0*	0.018*
L-cystine	3.0	3.0
Choline bitartrate	2.5	2.5
Tert-butylhydroquinone	0.014	0.014
Total Kcal/Kg	3760	3760

1Dyets, Inc. (Bethlehem, PA); ²Harlan Teklad (Madison, WI); ³Allied Food Service (Toronto, ON); ⁴Loblaws (Toronto, ON); ^†Fat in the RFol and HFol diets was derived from soybean oil.
*Denotes differences between diets.