Prevention of Osteoporosis in the Ovariectomized Rat by Oral Administration of a Nutraceutical Combination That Stimulates Nitric Oxide Production

Figure 7

Tartrate acid phosphatase (TRAP) staining in femur. Paraffin embedded sections of proximal epiphysis of the femora were stained with prewarmed TRAP staining solution, as in Figure 6. Top Panel: representative images of positive trap cells in (a): SHAM, (b): OVX, (c): OVX+E2, and (d): OVX+COMB-4 after 100 days of treatment. TRAP+ cells are stained in pink/violet. Sections were counterstained with hematoxylin to observe the cell nuclei. Magnification 400X; Bar: 50 um. Bottom Panel: number of TRAP-positive staining cells per field. No significant difference was observed among the groups. n = 8 per group.