The Senolytic Drug Fisetin Attenuates Bone Degeneration in the Zmpste24^−/− Progeria Mouse Model

Figure 1

Musculoskeletal abnormalities in Zmpste24^−/− (Z24^−/−) mice. (a) 4-month-old WT versus Z24^−/− mice indicating decreased weight and hunched posture. (b) Representative Alizarin red and Alcian blue staining of 4 mo WT and Z24^−/− skeletal preparations. (c) Micro-CT morphometry of hindlimbs indicating significant decrease in trabecular bone density in 4 mo Z24 mice versus age-matched WT (n = 6, ). (d) Representative radiographic images of Z24^−/− knee joint versus WT showing symptoms of OA. (e) Representative Safranin O staining highlighting spontaneous proteoglycan loss in articular cartilage of 4 mo Z24^−/− mice (n = 3, ). (f) Representative image of skeletal muscle section demonstrating senescent cell presence in WT versus Z24^−/− mice (n = 3, ).

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