The Senolytic Drug Fisetin Attenuates Bone Degeneration in the Zmpste24^−/− Progeria Mouse Model

Figure 2

Senolytic drugs reduce senescent cell number in MC3T3 osteoblasts and ATDC5 chondrocytes. Representative flow cytometry plots indicating gating strategy for MC3T3 and ATDC5 cells stained for 1 h with the florescent senescence marker C₁₂FDG following treatment with either DQ (200 nM + 20 μM) or FIS (50 μM) for 48 h. Unstained plots represent cells not exposed to C₁₂FDG used to exclude debris. Histograms indicating shift in % senescent cell population in cells treated with D + Q or FIS. Quantification reflects multiple experiments from passage (∼8) per cell type with 3 wells per treatment, .