Osteoblast-Specific Overexpression of Nucleolar Protein NO66/RIOX1 in Mouse Embryos Leads to Osteoporosis in Adult Mice

Figure 2

Immunostaining. (a), (b) (e), (f), immunohistochemistry. The femur sections of wild type (WT) (a), (e) and NO66 transgenic (TG) (b), (f) mice at E15.5 and P10 were stained with anti-FLAG antibody. The boxed area in (a) and (b) indicate periosteum. Red arrows in (b) and (f) point to cells expressing FLAG-tagged NO66 transgenic protein (dark brown nuclear staining). Os, ossification center; Hy, hypertrophic chondrocytes; Tr, trabeculae. (c), (d), immunofluorescence staining. The femur sections of WT (c) and TG (d) embryos at E18.5 were stained with anti-FLAG antibody (red). Cell nuclei were counterstained with DAPI (blue). Autofluorescence signal (green) within the ossification center (Os) indicates bone marrow. Yellow arrows in (d) point to cells expressing FLAG-tagged NO66 transgenic protein (red nuclear staining).