Osteoblast-Specific Overexpression of Nucleolar Protein NO66/RIOX1 in Mouse Embryos Leads to Osteoporosis in Adult Mice

Figure 3

RT-qPCR and histological assays. (a). The results of RT-qPCR assay for expression of the bone-differentiation marker genes Bsp, Col1a1, and Alp. (b)–(e) Histological examination. Alcian blue (b), (c) and von Kossa (d), (e) staining of the distal femoral sections of newborn (P1) wild type (WT) and NO66 transgenic mice. The red squares in (b) and (c) show hypertrophic zone of cartilage; brown staining signals in (d) and (e) indicate mineralized tissue.