Osteoblast-Specific Overexpression of Nucleolar Protein NO66/RIOX1 in Mouse Embryos Leads to Osteoporosis in Adult Mice

Figure 5

Histological examination and induction of adipogenic as well as osteogenic differentiation in ex vivo culture of bone marrow stromal cells (BMSC). (a), (b), H&E staining of the distal femur sections of three-month-old wild type (WT) and Col1a1-NO66 transgenic mice. Yellow arrows indicate fatty droplets within the bone marrow. Gp, growth plate; Tb, trabeculae. (c) Number of fatty droplets shown in (a) and (b). (d), (e) (g), (h), Oil Red (d), (e), and von Kossa (g), (h) staining of the primary cultured BMSC from long bones of three-month-old female WT and transgenic mice (n = 5). Blue arrows in (d) and (e) point to fatty droplets. (f) Number of fatty droplets shown in (d) and (e) (counted within three randomly selected image-fields). (i) Quantification of staining density shown in (g) and (h) (using ImageJ software) ().