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Journal of Pathogens
Volume 2011 (2011), Article ID 296275, 8 pages
http://dx.doi.org/10.4061/2011/296275
Research Article

A Selective Chromogenic Plate, YECA, for the Detection of Pathogenic Yersinia enterocolitica: Specificity, Sensitivity, and Capacity to Detect Pathogenic Y. enterocolitica from Pig Tonsils

Unité Hygiène et Qualité des Produits Avicoles et Porcins, Anses, BP 53, 22440 Ploufragan, France

Received 11 February 2011; Accepted 23 March 2011

Academic Editor: Latiful Bari

Copyright © 2011 M. Denis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. C. Savin and E. Carniel, “Les diarrhées d'origine bactérienne : le cas de Yersinia enterocolitica,” Revue Francophone des Laboratoires, vol. 38, no. 400, pp. 49–58, 2008. View at Google Scholar
  2. EFSA, “The community summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in the European Union in 2008,” EFSA Journal, vol. 8, no. 1, Article ID 1496, 410 pages, 2010. View at Publisher · View at Google Scholar
  3. EFSA, “Community report on trends & sources of zoonoses and zoonotic agents in the EU in 2007,” EFSA Journal, 223 pages, 2009. View at Google Scholar
  4. E. J. Bottone, “Yersinia enterocolitica: overview and epidemiologic correlates,” Microbes and Infection, vol. 1, no. 4, pp. 323–333, 1999. View at Publisher · View at Google Scholar
  5. G. Kapperud and O. Olsvik, “Isolation of enterotoxigenic Yersinia enterocolitica from birds in Norway,” Journal of Wildlife Diseases, vol. 18, no. 2, pp. 247–248, 1982. View at Google Scholar
  6. M. Lindblad, H. Lindmark, S. T. Lambertz, and R. Lindqvist, “Microbiological baseline study of broiler chickens at Swedish slaughterhousess,” Journal of Food Protection, vol. 69, no. 12, pp. 2875–2882, 2006. View at Google Scholar
  7. M. Bucher, C. Meyer, B. Grotzbach, S. Wacheck, A. Stolle, and M. Fredriksson-Ahomaa, “Epidemiological data on pathogenic Yersinia enterocolitica in Southern Germany during 2000–2006,” Foodborne Pathogens and Disease, vol. 5, no. 3, pp. 273–280, 2008. View at Publisher · View at Google Scholar
  8. S. T. Lambertz, C. Nilsson, S. Hallanvuo, and M. Lindblad, “Real-time PCR method for detection of pathogenic Yersinia enterocolitica in Food,” Applied and Environmental Microbiology, vol. 74, no. 19, pp. 6060–6067, 2008. View at Publisher · View at Google Scholar
  9. S. Bonardi, A. Paris, L. Bassi et al., “Detection, semiquantitative enumeration, and antimicrobial susceptibility of Yersinia enterocolitica in pork and chicken meats in Italy,” Journal of Food Protection, vol. 73, pp. 92–1785, 2010. View at Google Scholar
  10. S. M. Ostroff, G. Kapperud, L. C. Hutwagner et al., “Sources of sporadic Yersinia enterocolitica infections in Norway: a prospective case-control study,” Epidemiology and Infection, vol. 112, no. 1, pp. 133–141, 1994. View at Publisher · View at Google Scholar
  11. J. Fosse, H. Seegers, and C. Magras, “Prevalence and risk factors for bacterial food-borne zoonotic hazards in slaughter pigs: a review,” Zoonoses and Public Health, vol. 56, no. 8, pp. 429–454, 2009. View at Publisher · View at Google Scholar
  12. V. Thibodeau, E. H. Frost, S. Chénier, and S. Quessy, “Presence of Yersinia enterocolitica in tissues of orally-inoculated pigs and the tonsils and feces of pigs at slaughter,” Canadian Journal of Veterinary Research, vol. 63, no. 2, pp. 96–100, 1999. View at Google Scholar
  13. T. Nesbakken, K. Eckner, H. K. Hoidal, and O. J. Rotterud, “Occurrence of Yersinia enterocolitica and Campylobacter spp. in slaughter pigs and consequences for meat inspection, slaughtering, and dressing procedures,” International Journal of Food Microbiology, vol. 80, no. 3, pp. 231–240, 2003. View at Google Scholar
  14. E. Skjerve, B. Lium, B. Nielsen, and T. Nesbakken, “Control of Yersinia enterocolitica in pigs at herd level,” International Journal of Food Microbiology, vol. 45, no. 3, pp. 195–203, 1998. View at Publisher · View at Google Scholar
  15. M. Fredriksson-Ahomaa, J. Björkroth, S. Hielm, and H. Korkeala, “Prevalence and characterization of pathogenic Yersinia enterocolitica in pig tonsils from different slaughterhouses,” Food Microbiology, vol. 17, no. 1, pp. 93–101, 2000. View at Publisher · View at Google Scholar
  16. S. Bonardi, F. Brindani, G. Pizzin et al., “Detection of Salmonella spp., Yersinia enterocolitica and verocytotoxin-producing Escherichia coli O157 in pigs at slaughter in Italy,” International Journal of Food Microbiology, vol. 85, no. 1-2, pp. 101–110, 2003. View at Google Scholar
  17. M. Gürtler, T. Alter, S. Kasimir, M. Linnebur, and K. Fehlhaber, “Prevalence of Yersinia enterocolitica in fattening pigs,” Journal of Food Protection, vol. 68, no. 4, pp. 850–854, 2005. View at Google Scholar
  18. N. Kechagia, C. Nicolaou, V. Ioannidou et al., “Detection of chromosomal and plasmid—encoded virulence determinants in Yersinia enterocolitica and other Yersinia spp. isolated from food animals in Greece,” International Journal of Food Microbiology, vol. 118, no. 3, pp. 326–331, 2007. View at Google Scholar
  19. R. Laukkanen, P. O. Martinez, K. M. Siekkinen, J. Ranta, R. Maijala, and H. Korkeala, “Contamination of carcasses with human pathogenic Yersinia enterocolitica 4/O:3 originates from pigs infected on farms,” Foodborne Pathogens and Disease, vol. 6, no. 6, pp. 681–688, 2009. View at Publisher · View at Google Scholar
  20. I. Van Damme, I. Habib, and L. De Zutter, “Yersinia enterocolitica in slaughter pig tonsils: enumeration and detection by enrichment versus direct plating culture,” Food Microbiology, vol. 27, no. 1, pp. 158–161, 2010. View at Publisher · View at Google Scholar
  21. ISO 10273, Microbiology of Food and Animal Feeding Stuffs—Horizontal Method for the Detection of Presumptive Pathogenic Yersinia enterocolitica (ISO 10273:2003), International Organization for Standardization, Geneva, Switzerland, 2003.
  22. EFSA, “Scientific opinion of the panel on BIOHAZ on a request from EFSA on monitoring and identification of human enteropathogenic Yersinia spp,” The European Food Safety Authority, p. 30, 2007. View at Google Scholar
  23. E. De Boer, “Isolation of Yersinia enterocolitica from foods,” International Journal of Food Microbiology, pp. 75–84, 1992. View at Google Scholar
  24. M. Fondrevez, A. Labbé, E. Houard et al., “Simplified method for detecting pathogenic Yersinia enterocolitica in slaughtered pig tonsils,” Journal of Microbiological Methods, vol. 83, pp. 244–249, 2010. View at Publisher · View at Google Scholar
  25. S. D. Weagant, “A new chromogenic agar medium for detection of potentially virulent Yersinia enterocolitica,” Journal of Microbiological Methods, vol. 72, no. 2, pp. 185–190, 2008. View at Publisher · View at Google Scholar
  26. M. Fredriksson-Ahomaa, S. Wacheck, M. Koenig, A. Stolle, and R. Stephan, “Prevalence of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis pseudotuberculosis in wild boars in Switzerland,” International Journal of Food Microbiology, vol. 135, pp. 199–202, 2009. View at Publisher · View at Google Scholar
  27. M. P. Doyle and M. B. Hugdahl, “Improved procedure for recovery of Yersinia enterocolitica from meats,” Applied and Environmental Microbiology, vol. 45, pp. 127–135, 1983. View at Google Scholar
  28. G. Wauters, V. Goossens, M. Janssens, and J. Vandepitte, “New enrichment method for isolation of pathogenic Yersinia enterocolitica serogroup O:3 from pork,” Applied and Environmental Microbiology, vol. 54, pp. 851–854, 1988. View at Google Scholar
  29. J. Kwaga, J. O. Iversen, J. R. Saunders et al., “Comparison of two enrichment protocols for the detectionof Yersinia in slaughtered pigs and pork products,” Journal of Food Protection, vol. 53, pp. 1047–1049, 1990. View at Google Scholar
  30. E. De Boer and J. F. M. Nouws, “Slaughter pigs and pork as a source of human pathogenic Yersinia enterocolitica,” International Journal of Food Microbiology, vol. 12, pp. 375–378, 1991. View at Publisher · View at Google Scholar
  31. L. De Zutter, L. Le Mort, M. Janssens, and G. Wauters, “Short-comings of irgasan titarcillin chlorate broth for the enrichment of Yersinia enterocolitica biotype 2, serotype 9 from meat,” International Journal of Food Microbiology, vol. 23, pp. 231–237, 1994. View at Google Scholar
  32. A. Leclercq, “Le genre Yersinia et son incidence dans le domaine alimentaire,” Cours de l'Institut Pasteurde Lille. Institut Pasteur de Lille, 2003.
  33. D. A. Schiemann, “Synthesis of a selective agar medium for Yersinia enterocolitica,” Canadian Journal of Microbiology, vol. 25, pp. 1298–1304, 1979. View at Publisher · View at Google Scholar
  34. D. A. Schiemann , “Comparison of enrichment and plating media for recovery of virulent strains of Yersinia enterocolitica from inoculated beef stew,” Journal of Food Protection, vol. 46, pp. 957–964, 1983. View at Google Scholar
  35. S. J. Walker and A. Gilmour, “The incidence of Yersinia enterocolitica and Yersinia enterocolitica-like organismsin raw and pasteurised milk in Northern Ireland,” The Journal of Applied Bacteriology, vol. 61, pp. 133–138, 1986. View at Google Scholar
  36. N. A. Cox, J. S. Bailey, F. Del Corral, and E. B. Shotts, “Comparison of enrichment and plating media for isolation of Yersinia,” Poultry Science, vol. 69, no. 4, pp. 686–693, 1990. View at Google Scholar
  37. J. A. Devenish and D. A. Schiemann, “An abbreviated scheme for identification of Yersinia enterocolitica isolated from food enrichment on CIN (cefsulodin-irgasannovobiocin) agar,” Canadian Journal of Microbiology, vol. 27, pp. 937–941, 1981. View at Publisher · View at Google Scholar
  38. M. C. Harmon, C. L. Yu, and B. Swaminathan, “An evaluation of selective differential plating media forthe isolation of Yersinia enterocolitica from experimentally inoculated fresh ground pork homogenate,” Journal of Food Science, vol. 48, no. 1, pp. 6–9.
  39. A. Von Altrock, A. L. Louis, U. Rosler et al., “The bacteriological and serological prevalence of Campylobacter spp and Yersinia enterocolitica fattening pig herds in Lower Saxony,” Berliner und Munchener Tierarztliche Wochenschrift, vol. 119, pp. 391–399, 2006. View at Google Scholar
  40. A. S. Milnes, I. Stewart, F. A. Clifton-Hadley et al., “Intestinal carriage of verocytotoxigenic Escherichia coli, Salmonella, thermophilic Campylobacter and Yersinia enterocolitica, in cattle, sheep and pigs at slaughter in Great Britain during 2003,” Epidemiology and Infection, vol. 136, pp. 739–751, 2008. View at Google Scholar
  41. EFSA and the Members of the group of work Yersinia enterocolitica, “Technical specifications for harmonized national surveys on Yersinia enterocolitica in slaughter pigs,” EFSA Journal, vol. 7, no. 11, Article ID 1374, 32 pages, 2009. View at Publisher · View at Google Scholar
  42. D. A. Schiemann, “Development of a two-step enrichment procedure for recovery of Yersinia enterocolitica from food,” Applied and Environmental Microbiology, vol. 43, pp. 14–27, 1982. View at Google Scholar