Virulence Plasmid (pYV)-Associated Expression of Phenotypic Virulent Determinants in Pathogenic Yersinia Species: A Convenient Method for Monitoring the Presence of pYV under Culture Conditions and Its Application for Isolation/Detection of Yersinia pestis in Food

Table 2

Effect of media on CR-uptake in pYV-bearing Y. enterocolitica, Y. pseudotuberculosis, and Y. pestis (adapted from [7]).

Organism^a	Strain	CR-BHO	CR-TSO	CR-MOX
Y. enterocolitica	GER	+	+	+
Y. enterocolitica-RE	GER	+	+	+
Y. enterocolitica-C	GER	−	−	−
Y. pseudotuberculosis	PB1/+	+	+	+
Y. pseudotuberculosis-RE	PB1/+	+	+	+
Y. pseudotuberculosis-C	PB1/+	−	−	−
Y. pestis	KIM5	−	−	+
Y. pestis-RE	KIM5	−	−	−
Y. pestis-C	KIM5	−	−	−
pYV-less Y. pestis	Kuma	−	−	−

^aCells recovered from red pinpoint colonies and subcultured in BHI broth at 28°C are designated as RE. The pYV-negative strains of Y. enterocolitica Y. pseudotuberculosis, and Y. pestis are designated as C (cured).
Low-calcium: CR-BHO (238 μM Ca²⁺) and CR-TSO (311 μM Ca²⁺). CR-MOX (calcium deficient).