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Journal of Pathogens
Volume 2012 (2012), Article ID 357235, 4 pages
Research Article

Identification at Biovar Level of Brucella Isolates Causing Abortion in Small Ruminants of Iran

1Brucellosis Department, Razi Vaccine and Serum Research Institute, Karaj 3197619751, Iran
2Office of Brucellosis Control and Eradication, Iranian Veterinary Organization, Tehran 1595814111, Iran
3Food Hygiene and Quality Control Department, Faculty of Veterinary Medicine, University of Tehran, Tehran 1419963111, Iran

Received 29 August 2012; Accepted 31 October 2012

Academic Editor: Hin-Chung Wong

Copyright © 2012 Ali Mohammad Behroozikhah et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


To determine the most prevalent biovar responsible for brucellosis in sheep and goat populations of Iran, a cross-sectional study was carried out over 2 years in six provinces selected based on geography and disease prevalence. Specimens obtained from referred aborted sheep and goat fetuses were cultured on Brucella selective media for microbiological isolation. Brucellae were isolated from 265 fetuses and examined for biovar identification using standard microbiological methods. Results showed that 246 isolates (92.8%) were B. melitensis biovar 1, 18 isolates (6.8%) were B. melitensis biovar 2, and, interestingly, one isolate (0.4%) obtained from Mazandaran province was B. abortus biovar 3. In this study, B. melitensis biovar 3 was isolated in none of the selected provinces, and all isolates from 3 provinces (i.e., Chehar-mahal Bakhtiari, Markazi, and Ilam) were identified only as B. melitensis biovar 1. In conclusion, we found that B. melitensis biovar 1 remains the most prevalent cause of small ruminant brucellosis in various provinces of Iran.