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Journal of Pathogens
Volume 2013, Article ID 194932, 10 pages
http://dx.doi.org/10.1155/2013/194932
Clinical Study

Monitoring of Active Human Herpes Virus 6 Infection in Iranian Patients with Different Subtypes of Multiple Sclerosis

1Department of Neurology, Zahedan University of Medical Science, Zahedan 43181-98168-43175, Iran
2Department of Biology, Faculty of Science, Zabol University, Zabol 98613335856, Iran
3Cellular and Molecular Research Center, Tehran University of Medical Sciences, Hemmat Campus, Tehran 1449614535, Iran
4Department of Pathology, Zahedan University of Medical Sciences, Zahedan 43181-98168-43175, Iran

Received 25 July 2012; Revised 19 October 2012; Accepted 2 November 2012

Academic Editor: Timothy J. Johnson

Copyright © 2013 Nourollah Ramroodi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Recently, it has been suggested that human herpes virus 6 (HHV6) may play a role in the pathogenesis of multiple sclerosis (MS). Our purpose is to determine the incidence of reactivated HHV6 in MS patients. Methods. Viral sequence analyzed by qPCR in the peripheral blood mononuclear cells (PBMCs), serum, and saliva samples of different subtypes of MS patients ( ) and healthy controls ( ). HHV6 IgG and IgM antibody levels measured by ELISA technique in the plasma samples of both groups. Likewise, cerebrospinal fluid (CSF) samples of some MS patients ( ) were analyzed for viral sequence. Results. Results demonstrate increased levels of anti-HHV6-IgG (78.2% versus 76.4% in controls; ), and IgM (34.6% versus 6.5% in controls; ) in MS patients. Furthermore, RRMS and SPMS patients showed relatively higher anti-HHV6 IgG and IgM compared to PPMS ( ). Moreover, load of cell-free viral DNA was higher in RRMS and SPMS patients and detected in 60.2% (47/78) of MS patients, compared with 14.6% (18/123) of healthy controls ( ). Moreover, load of cell-free viral DNA was higher in RRMS and SPMS patients and detected in 60.2% (47/78) of MS patients, compared with 14.6% (18/123) of healthy controls ( ). Conclusions. The results extend the observation of an increased frequency of systemic reactivated HHV6 infection in MS patients with developed stages of disease.