Proof of Principle for a Real-Time Pathogen Isolation Media Diagnostic: The Use of Laser-Induced Breakdown Spectroscopy to Discriminate Bacterial Pathogens and Antimicrobial-Resistant Staphylococcus aureus Strains Grown on Blood Agar
Table 2
Mutations detected in MM66 and SH1000-1 by comparative genome sequencing.
Strains
SACOL locia
Gene
Function
SNP positiona
Amino acid changea
SH1000-1
Intragenic
SACOL0593
fusA
Elongation factor G
C617228 T617228
H457 Y457
SACOL0358
A371671 T371671
N36 I36
T317672 A371672
N36 I36
G371676 A371676
E37 K37
A371685 C371685
K40 Q40
MM66-4
Intragenic
SACOL1883-
Hypothetical protein
C1938819 T1938819
TRNA-ser
tRNA-ser
T1938825 A1938825
SACOL1947-
Hypothetical protein
C2010604 T2010604
SACOL1948
Hypothetical protein
A2010605 G2010605
SACOL2575-
Putative aromatic Amino transferase
A2638759 T2638759
SACOL2576
crtN
Squalene synthase
C2638762A2638762
Intragenic
SACOL1690
apt
Adenine phosphoribosyl- transferase
C1721075 T1721075
A57 V57
SACOL0020
yycG
Sensory box histidine kinase
A26449 G26449
K263 E263
SNP: single nucleotide polymorphism. Based on loci numbers, nucleotide positions, and amino acid residues in NCBI Genbank database S. aureus strain COL reference genome.