Isolation of Environmental Bacteria from Surface and Drinking Water in Mafikeng, South Africa, and Characterization Using Their Antibiotic Resistance Profiles
Table 1
Oligonucleotide primers that were used for specific detection of Pseudomonas species.
Primer
Oligonucleotide sequence (5′-3′)
Target and size (pb)
PCR cycling conditions
ECF1 ECF2
ATGGATGAGCGCTTCCGTG TCATCCTTGCCTCCCTG
ecfX, (528)
35X 94°C for 45 s 58°C for45 s 72°C for 1 m
GyrPA-398 GyrPA-620
CCTGACCATCCGTCGCCACAAC CGCAGCAGGATGCCGACGCC
gyrB, (222)
35X 94°C for 45 s 66°C for45 s 72°C for 1 m
ETA1 ETA2
GACAACGCCCTCAGCATCACCAGC CGCTGGCCCATTCGCTCCAGCGCT
toxA, (367)
35X 94°C for 45 s 66°C for45 s 72°C for 1 m
Initial denaturing step of 95°C for 5 min and final strand extension of 72°C for 5 min.
