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Journal of Pathogens
Volume 2014, Article ID 407385, 5 pages
Research Article

Detection of Legionella spp. from Domestic Water in the Prefecture of Arta, Greece

1Department of Microbiology, National School of Public Health, 196 Alexandras Avenue, 115 21 Athens, Greece
2Central Public Health Laboratory, 34 Alexandrou Fleming, Vari, 16672 Attika, Greece

Received 25 November 2013; Accepted 7 February 2014; Published 12 March 2014

Academic Editor: Mariela A. Segura

Copyright © 2014 Dimitra Dimitriadi and Emmanuel Velonakis. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this research was the isolation of Legionella spp. from domestic water supply networks in the Prefecture of Arta. A total of 100 water samples, from 25 houses, were collected. Half of the samples concerned the cold water and half the hot water supply. Purpose was to detect colonization of the water networks with Legionella spp. >500 cfu/L by using the method of filtration (ISO 11731). Out of 100 samples, 6 samples from 3 houses were positive for Legionella spp. Legionella pneumophila serogroup 2–14 was isolated in 5 of 6 samples, whereas in the sixth sample Legionella anisa was identified. Only three of the samples had residual chloride over 0.2 mg/L, rate which is necessary for potable water, according to the Greek hygienic practice. Concerning the temperature of hot water, the mean temperature of the negative for Legionella samples was higher compared to the mean temperature of the positive for Legionella samples (49.9°C versus 45.5°C). It is estimated that there is risk of infection through the use of showers. The low concentration of chloride and the temperature, which was found within the limits favorable to developing Legionella spp. (20–45°C), provide fertile ground for proliferation of the bacteria.