Table of Contents Author Guidelines Submit a Manuscript
Journal of Pathogens
Volume 2015, Article ID 516869, 5 pages
Research Article

Evaluation of In Vivo Acaricidal Effect of Soap Containing Essential Oil of Chenopodium ambrosioides Leaves on Rhipicephalus lunulatus in the Western Highland of Cameroon

1Department of Animal Production, Faculty of Agronomy and Agricultural Sciences, University of Dschang, P.O. Box 222, Dschang, Cameroon
2Center for Research on Filariasis and Other Tropical Diseases (CRFilMT), P.O. Box 5797, Yaoundé, Cameroon
3Department of Animal Sciences, Faculty of Sciences, University of Dschang, P.O. Box 67, Dschang, Cameroon
4Higher National Institute of Agronomy and Biotechnology (INSAB), University of Sciences and Techniques of Masuku, P.O. Box 941, Franceville, Gabon

Received 26 May 2015; Revised 16 September 2015; Accepted 7 October 2015

Academic Editor: Alexander Idnurm

Copyright © 2015 Marc K. Kouam et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A study on the acaricidal properties of foam soap containing the essential oil of Chenopodium ambrosioides leaves was carried out on Rhipicephalus lunulatus. Four doses (0.03, 0.06, 0.09, and 0.12 µL of essential oil per gram of soap) and a control (soap without essential oil) with four replications for each treatment were used for in vitro trial. Each replication consisted of 10 ticks in a Petri dish with filter paper impregnated with the foam soap on the bottom. Following in vitro trials, three doses (0.06, 0.09, and 0.12 µL/g) and the control in two replications were selected for in vivo test based on mortality rate recorded from the in vitro trial. Each replication was made up of 10 goats naturally infested with ticks. Results show that soap containing essential oil is toxic to R. lunulatus. The in vivo mortality rate in the control on day 8 was 22.69% whereas the highest dose (0.12 µL/g) killed 96.29% of the ticks on day 8. The LD50 of the foam soap containing essential oil was 0.037 and 0.059 µL/g on day 2 in the laboratory and on the farm, respectively. This indicates the potentially high efficiency of this medicated soap on this parasite.