Restriction Profiling of 23S Microheterogenic Ribosomal Repeats for Detection and Characterizing of E. coli and Their Clonal, Pathogenic, and Phylogroups

<div>(a) Broad Range PCR of 23S P1880 with genomic DNA isolated from standard strains. Lane 1: 100 bp ladder; Lane 2: blank; Lane 3: E. coli; Lane 4: C. freundii; Lane 5: C. koseri; Lane 6: K. pneumonia; Lane 7: E. aerogenes. PCR product of single representative of E. coli was loaded. (b) ARDRA of 23S P1880 amplicons with Bfa I: Lanes 1, 2, and 3: E. coli (ATCC15223, ATCC25922, and ATCC11775); Lane 4: C. freundii (ATCC8090); Lane 5: C. koseri (ATCC27028); Lane 6: K. pneumonia (ATCC33495); Lane 7: E. aerogenes (ATCC13048); Lane 8: 100 bp ladder.</div>

Journal of Pathogens

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Figure 2: Restriction Profiling of 23S Microheterogenic Ribosomal Repeats for Detection and Characterizing of E. coli and Their Clonal, Pathogenic, and Phylogroups

Figure 2 | Restriction Profiling of 23S Microheterogenic Ribosomal Repeats for Detection and Characterizing of E. coli and Their Clonal, Pathogenic, and Phylogroups 