Research Article
Restriction Profiling of 23S Microheterogenic Ribosomal Repeats for Detection and Characterizing of E. coli and Their Clonal, Pathogenic, and Phylogroups
Table 2
Restriction site of Bfa I (C/TAG) within 23S rrlH of candidate and noncandidates and the restriction profiles with 23S P1880.
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Primers were designed against the regions flanking the bold restriction sites. F: number of fragments; E: Escherichia coli; Ci: Citrobacter koseri; K: Klebsiella pneumoniae; Ea: Enterobacter aerogenes; Mic.: microbe. 23S P1880-FP(+): 5′-GGCGAAAAGAACCCCGGCGA-3′ (20 nt); 23S P1880-RP(−): 5′-AGGGGTCGACTCACCCTGCC-3′ (20 nt). FP: forward primer; RP: reverse primer. The primer details were obtained from the results of primer blast and restriction profiling pattern was obtained from NEB V2.0. Forward and reverse primers of 23S P1880 lie within domains I and III of 23S rRNA secondary structure, respectively, with Bfa I sites in domain II. |