Iron Deprivation Affects Drug Susceptibilities of Mycobacteria Targeting Membrane Integrity

Figure 1

Assessment of mycobacterial growth in response to iron deprivation. (a) Drug susceptibility assays against M. smegmatis in the presence of 2,2, BP. Left panel shows broth microdilution assay to determine the MIC₈₀ of M. smegmatis in the presence of 2,2, BP. The minimum drug concentration that inhibits growth by 80% relative to the drug-free growth control is indicated as MIC₈₀. Right panel shows spot assay of M. smegmatis in the absence (control) and the presence of subinhibitory concentration of 2,2, BP (35 μg/mL). (b) Growth curve of M. smegmatis in the presence of 35 μg/mL 2,2, BP.

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