Iron Deprivation Affects Drug Susceptibilities of Mycobacteria Targeting Membrane Integrity

Figure 3

Effect of iron supplementation on reversion of drug susceptibilities. Spot assays of iron deprived cells (2,2, BP, DFO, and BPS) at concentrations of 35 μg/mL, 656 μg/mL, and 386 μg/mL showing hypersensitivity with EMB, INH, and RIF and upon iron supplementation (FeCl₃) of 0.2 mM for 2,2, BP, 0.3 mM for BPS, and 0.4 mM for DFO showing rescuing of the drug susceptibilities of EMB, INH, and RIF (62.5 ng/mL, 1 μg/mL, and 62.5 ng/mL).