Iron Deprivation Affects Drug Susceptibilities of Mycobacteria Targeting Membrane Integrity

Figure 4

Effect of iron deprivation on cell membrane. (a) Nitrocefin membrane permeability assay for M. smegmatis cells grown in the absence (control) and the presence of 2,2, BP (35 μg/mL). Means of O.D₄₈₅ ± SD of three independent sets of experiments are depicted on -axis with respect to time (minutes) on -axis (). (b) Spot assay of M. smegmatis in the absence (control) and the presence of 2,2, BP (35 μg/mL) and cell membrane perturbing agent (SDS) at 0.025%.

(a)

(b)