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Journal of Pathogens
Volume 2016, Article ID 6235618, 8 pages
http://dx.doi.org/10.1155/2016/6235618
Research Article

Primary Multidrug Resistant Tuberculosis and Utility of Line Probe Assay for Its Detection in Smear-Positive Sputum Samples in a Tertiary Care Hospital in South India

Department of Microbiology, Government Medical College, Kozhikode, Kerala 673008, India

Received 16 October 2015; Revised 5 January 2016; Accepted 2 March 2016

Academic Editor: Abhineet S. Sheoran

Copyright © 2016 Fahmiya Leena Yacoob et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. World Health Organization, Global Tuberculosis Report 2015, World Health Organization, Geneva, Switzerland, 2015.
  2. Regional Office for South-East Asia, Tuberculosis in South East Asia Region, Annual TB Report 2015, World Health Organization-SEAR, New Delhi, India, 2015.
  3. Drug-Resistant TB, “Surveillance and response, supplement,” Global TB Report, World Health Organization, Geneva, Switzerland, 2014. View at Google Scholar
  4. WHO, “Molecular line probe assays for rapid screening of patients at risk of MDR-TB,” Policy Statement, WHO, 2008, http://www.who.int/tb/features_archive/policy_statement.pdf. View at Google Scholar
  5. TB India, Revised National Tuberculosis Control Programme Annual Status Report, Government of India, New Delhi, India, 2014.
  6. GenoType MTBDRplus—Hain Lifescience Ver 2.0, Instructions for use, IFU-304A-02, 2012.
  7. Training Module for Medical Practitioners, Central TB Division, Directorate General of Health Services, Ministry of Health and Family Welfare, Central TB Division, Revised National Tuberculosis Control Programme, 2010.
  8. C. Y. Jeon and M. B. Murray, “Diabetes mellitus increases the risk of active tuberculosis: a systematic review of 13 observational studies,” PLoS Medicine, vol. 5, no. 7, pp. 1091–1101, 2008. View at Publisher · View at Google Scholar · View at Scopus
  9. S. K. Sharma, G. Kaushik, B. Jha et al., “Prevalence of multidrug-resistant tuberculosis among newly diagnosed cases of sputum-positive pulmonary tuberculosis,” Indian Journal of Medical Research, vol. 133, no. 3, pp. 308–311, 2011. View at Google Scholar · View at Scopus
  10. M. R. Joseph, C. T. Shoby, G. R. Amma, L. S. Chauhan, and C. N. Paramasivan, “Surveillance of anti-tuberculosis drug resistance in Ernakulam District, Kerala State, South India,” International Journal of Tuberculosis and Lung Disease, vol. 11, no. 4, pp. 443–449, 2007. View at Google Scholar · View at Scopus
  11. D. T. B. D'Souza, N. F. Mistry, T. S. Vira et al., “High levels of multidrug resistant tuberculosis in new and treatment-failure patients from the revised national tuberculosis control programme in an urban metropolis (Mumbai) in Western India,” BMC Public Health, vol. 9, article 211, 2009. View at Publisher · View at Google Scholar · View at Scopus
  12. S. Sharma, M. Madan, C. Agrawal, and A. K. Asthana, “Genotype MTBDR plus assay for molecular detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis,” Indian Journal of Pathology and Microbiology, vol. 57, no. 3, pp. 423–426, 2014. View at Publisher · View at Google Scholar · View at Scopus
  13. N. Mohan, P. B. Chandrasekhar, I. J. Padmaja, N. Raizada, P. S. Rao, and B. S. Kumar, “Genotype MTBDRplus line probe assay for rapid and direct detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis complex from sputum samples,” Journal of Dr. NTR University of Health Sciences, vol. 3, pp. 23–27, 2014. View at Google Scholar
  14. D. Hillemann, S. Rüsch-Gerdes, and E. Richter, “Evaluation of the GenoType MTBDRplus assay for rifampin and isoniazid susceptibility testing of Mycobacterium tuberculosis strains and clinical specimens,” Journal of Clinical Microbiology, vol. 45, no. 8, pp. 2635–2640, 2007. View at Publisher · View at Google Scholar · View at Scopus
  15. M. A. L. Lingala, A. Srikantam, S. Jain, K. V. S. M. Rao, and P. V. Ranganadha Rao, “Clinical and geographical profiles of rpoB gene mutations in Mycobacterium tuberculosis isolates from Hyderabad and Koraput in India,” Journal of Microbiology and Antimicrobials, vol. 2, no. 2, pp. 13–18, 2010. View at Google Scholar
  16. P. Deepa, K. L. Therese, and H. N. Madhavan, “Detection and characterization of mutations in rifampicin resistant Mycobacterium tuberculosis clinical isolates by DNA sequencing,” Indian Journal of Tuberculosis, vol. 52, pp. 132–136, 2005. View at Google Scholar
  17. F. A. Drobniewski and S. M. Wilson, “The rapid diagnosis of isoniazid and rifampicin resistance in Mycobacterium tuberculosis-a molecular story,” Journal of Medical Microbiology, vol. 47, no. 3, pp. 189–196, 1998. View at Publisher · View at Google Scholar · View at Scopus
  18. M. Arentz, B. Sorensen, D. J. Horne, and J. L. Walson, “Systematic review of the performance of rapid rifampicin resistance testing for drug-resistant tuberculosis,” PLoS ONE, vol. 8, no. 10, Article ID e76533, 2013. View at Publisher · View at Google Scholar · View at Scopus
  19. P. Kumar, V. Balooni, B. K. Sharma, V. Kapil, K. S. Sachdeva, and S. Singh, “High degree of multi-drug resistance and hetero-resistance in pulmonary TB patients from Punjab state of India,” Tuberculosis, vol. 94, no. 1, pp. 73–80, 2014. View at Publisher · View at Google Scholar · View at Scopus
  20. S. Hofmann-Thiel, J. Van Ingen, K. Feldmann et al., “Mechanisms of heteroresistance to isoniazid and rifampin of Mycobacterium tuberculosis in Tashkent, Uzbekistan,” European Respiratory Journal, vol. 33, no. 2, pp. 368–374, 2009. View at Publisher · View at Google Scholar · View at Scopus
  21. M. P. Tolani, D. T. B. D'souza, and N. F. Mistry, “Drug resistance mutations and heteroresistance detected using the GenoType MTBDRplus assay and their implication for treatment outcomes in patients from Mumbai, India,” BMC Infectious Diseases, vol. 12, article 9, 2012. View at Publisher · View at Google Scholar · View at Scopus
  22. I. Mokrousov, O. Narvskaya, T. Otten, E. Limeschenko, L. Steklova, and B. Vyshnevskiy, “High prevalence of KatG Ser315Thr substitution among isoniazid-resistant Mycobacterium tuberculosis clinical isolates from northwestern Russia, 1996 to 2001,” Antimicrobial Agents and Chemotherapy, vol. 46, no. 5, pp. 1417–1424, 2002. View at Publisher · View at Google Scholar · View at Scopus
  23. A. Van Rie, R. Warren, I. Mshanga et al., “Analysis for a limited number of gene codons can predict drug resistance of Mycobacterium tuberculosis in a high-incidence community,” Journal of Clinical Microbiology, vol. 39, no. 2, pp. 636–641, 2001. View at Publisher · View at Google Scholar · View at Scopus
  24. A. K. Maurya, J. Umrao, A. K. Singh, S. Kant, R. A. Kushwaha, and T. N. Dhole, “Evaluation of GenoType MTBDRplus assay for rapid detection of drug susceptibility testing of multi-drug resistance tuberculosis in Northern India,” Indian Journal of Pathology and Microbiology, vol. 56, no. 2, pp. 139–143, 2013. View at Publisher · View at Google Scholar
  25. M. Veluchamy, R. Madhavan, S. Narayanan, and L. Rajesh, “KatG gene as a surrogate molecular marker leading to cause drug resistance in Mycobacterium tuberculosis isolates,” American Journal of Infectious Diseases and Microbiology, vol. 1, no. 5, pp. 86–91, 2013. View at Publisher · View at Google Scholar
  26. M. N. T. Huyen, F. G. J. Cobelens, T. N. Buu et al., “Epidemiology of isoniazid resistance mutations and their effect on tuberculosis treatment outcomes,” Antimicrobial Agents and Chemotherapy, vol. 57, no. 8, pp. 3620–3627, 2013. View at Publisher · View at Google Scholar · View at Scopus
  27. H. Ohno, H. Koga, S. Kohno, T. Tashiro, and K. Hara, “Relationship between rifampin MICs for and rpoB mutations of Mycobacterium tuberculosis strains isolated in Japan,” Antimicrobial Agents and Chemotherapy, vol. 40, no. 4, pp. 1053–1056, 1996. View at Google Scholar · View at Scopus
  28. R. Raveendran, C. Wattal, J. K. Oberoi, N. Goel, S. Datta, and K. J. Prasad, “Utility of GenoType MTBDRplus assay in rapid diagnosis of multidrug resistant tuberculosis at a tertiary care centre in India,” Indian Journal of Medical Microbiology, vol. 30, no. 1, pp. 58–63, 2012. View at Publisher · View at Google Scholar · View at Scopus
  29. R. Anek-vorapong, C. Sinthuwattanawibool, L. J. Podewils et al., “Validation of the GenoType® MTBDRplus assay for detection of MDR-TB in a public health laboratory in Thailand,” BMC Infectious Diseases, vol. 10, article 123, 2010. View at Publisher · View at Google Scholar · View at Scopus
  30. S. K. Patra and A. Jain, “Molecular diagnosis of multi drug resistant tuberculosis,” International Journal of Biomedical and Advance Research, vol. 3, no. 5, pp. 273–280, 2012. View at Publisher · View at Google Scholar