Analysis of Aminoglycoside Modifying Enzyme Genes Responsible for High-Level Aminoglycoside Resistance among Enterococcal Isolates

Table 1

Primers used in the multiplex PCR [9].

Aminoglycoside resistance gene	Product size (bp)	Sequence type	Primer sequence ()
aac(6′)-Ie-aph(2′′)-Ia	369	Fw	CAGGAATTTATCGAAAATGGTAGAAAAG
		R	CACAATCGACTAAAGAGTACCAATC
aph(2′′)-Ib	867	Fw	CTTGGACGCTGAGATATATGAGCAC
		R	GTTTGTAGCAATTCAGAAACACCCTT
aph(2′′)-Ic	444	Fw	CCACAATGATAATGACTCAGTTCCC
		R	CCACAGCTTCCGATAGCAAGAG
aph(2′′)-Id	641	Fw	GTGGTTTTTACAGGAATGCCATC
		R	CCCTCTTCATACCAATCCATATAACC
aph(3′)-IIIa	523	Fw	GGCTAAAATGAGAATATCACCGG
		R	CTTTAAAAAATCATACAGCTCGCG
ant(4′)-Ia	294	Fw	CAAACTGCTAAATCGGTAGAAGCC
		R	GGAAAGTTGACCAGACATTACGAACT

Fw: forward primer; R: reverse primer.