Research Article
Temporal Regulation of fim Genes in Uropathogenic Escherichia coli during Infection of the Murine Urinary Tract
Table 1
Bacterial strains and plasmids used in this study.
| | Strains/plasmids | Description | Reference or source |
| | E. coli strains | | | | NU149 | Cystitis clinical isolate | [30] | | DH5α MCR | General cloning strain | Gibco/BBL | | Plasmids | | | | pAON-1 | fimA-lacZYA locked Phase-ON on pUJ8 | [28] | | pP5-48 | fimB-lacZYA on pUJ8 | [28] | | pMP5-2.17 | fimE-lacZYA on pUJ8 | [28] | | pUJ8 | - phoA Ap | [42] | | pHSS22 | oriT Km | [28] | | pXen-5 | Emr, promoterless lux operon | [43] | | pPP2-6 | pPR274 with multiple cloning site and Cm | [28] | | pWS141-2 | fimB promoters on pHSS22 | This study | | pWS144-27 | fimB-lux fusion on pHSS22 | This study | | pWS145-38 | fimB-lux fusion on pPP2-6 | This study | | pHD-01 | fimA promoter on pHSS22 | This study | | pHD-02 | fimA-lux fusion on pHSS22 | This study | | pHD-03 | fimA-lux fusion on pPP2-6 | This study | | pHD-04 | fimE promoter on pHSS22 | This study | | pHD-05 | fimE-lux fusion on pHSS22 | This study | | pHD-06 | fimE-lux fusion on pPP2-6 | This study | | pHD-07 | ftsZ promoter on pHSS22 | This study | | pHD-08 | ftsZ-lux fusion on pHSS22 | This study | | pHD-09 | ftsZ-lux fusion on pPP2-6 | This study |
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