Propagation and Molecular Characterization of Bioreactor Adapted Very Virulent Infectious Bursal Disease Virus Isolates of Malaysia

Figure 3

Gel electrophoresis of bioreactor passaged viruses. RT-PCR product showing 643 bp fragment of bioreactor passaged vvIBDV UPM0081EP12BGMP15BP1 (lane 2), UPM0081EP8BGMP1BP1 (lane 3), UPM190EP12BGMP15BP1 (lane 4), UMP190EP8BGMP1BP1 (lane 5), and original BGM-70 and egg passaged isolates UPM0081BGMP15 (lane 6), UPM190BGMP15 (lane 7), UPM0081EP8 (lane 8), UPM190EP8 (lane 9), UPM0081EP12 (lanes 10 and 11), UPM190EP12 (lanes 12 and 13), positive controls (lanes 14, 15, and 16), and negative control (lane 17). A 1000 bp DNA ladder (lanes 1 and 15) (MBI Fermentas, Lithuania) was used to flank the PCR products.