Propagation and Molecular Characterization of Bioreactor Adapted Very Virulent Infectious Bursal Disease Virus Isolates of Malaysia

Figure 7

The indirect immunofluorescence test on the bioreactor propagated UPM0081EP12BGMP15BP1 and UPM190EP12BGMP15BP1 showing high amount of IBDV antigen within the cytoplasm of BGM-70 cells at 72 hours after inoculation. ((a) to (c)) BGM-70 cells infected with bioreactor propagated UPM0081EP12BGMP15BP1 and ((d) to (f)) UPM190EP12BGMP15BP1 showing positive signals for VP2 antigen (green) when stained with FITC-conjugated anti-chicken antibody raised against chicken anti-VP2 antibody. ((g) to (i)) Uninoculated control. Bar=50μm.

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