Propagation and Molecular Characterization of Bioreactor Adapted Very Virulent Infectious Bursal Disease Virus Isolates of Malaysia

Figure 8

The indirect immunofluorescence test on the flask propagated UPM0081BGMP15 and UPM190BGMP15 showing low amount of IBDV antigen within the cytoplasm of infected BGM-70 cells at 72 hours after inoculation. ((a) to (c)) BGM-70 cells infected with flask propagated UPM0081BGMP15 and ((d) to (f)) UPM190BGMP15 showing positive signals for VP2 antigen (green) when stained with FITC-conjugated anti-chicken antibody raised against chicken anti-VP2 antibody. Bar=50 μm.

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